Faculty of Chemical and Metallurgical Engineering, Department of Food Engineering, Yildiz Technical University, Istanbul, Turkey.
TUBITAK Marmara Research Centre, Life Sciences, Kocaeli, Turkey.
J Food Sci. 2024 Sep;89(9):5335-5349. doi: 10.1111/1750-3841.17261. Epub 2024 Jul 23.
Inulin, a prebiotic utilized in the food and pharmaceutical industries, promotes the growth of beneficial bacteria in the colon, thereby enhancing human health. Although inulin is commercially produced from chicory and artichoke, Inula helenium roots offer a high potential for inulin production. The aim of this study is to investigate the prebiotic activity of inulin (inulin-P) from I. helenium roots on Lactobacillus rhamnosus, as well as its ability to produce synbiotic microcapsules and the effects on probiotic viability during freeze-drying, in vitro gastrointestinal (GI) digestion, and storage. First, the effect of inulin-P on L. rhamnosus viability and short-chain fatty acid (SCFA) production was compared to other commonly utilized prebiotics. The findings revealed that inulin-P remarkably promoted the growth and SCFA yield of L. rhamnosus for 48 h of fermentation and 28 days of storage. Then, L. rhamnosus was encapsulated with inulin-P and commercial inulin to compare its survival throughout storage and the GI tract. Inulin-P microcapsules outperformed in terms of viability during storage (7.98 log CFU/g after 30 days at 4°C). Furthermore, inulin-P microcapsules were heat-resistant and protected L. rhamnosus from GI conditions, resulting in a high survival rate (89.52%) following large intestine simulation, which is ideal for increasing customer benefits. Additionally, inulin-P microcapsules exhibited similar physical characteristics to commercial inulin. Consequently, this study revealed that inulin-P, which is easy to produce, low-cost, and has industrial application potential, could be used as a good carrier for the synbiotic encapsulation of L. rhamnosus. PRACTICAL APPLICATION: Inulin is a prebiotic that promotes the activity and growth of beneficial bacteria in the human gut. Although commercial inulin is currently produced from chicory root and artichoke, Inula helenium root is a potential raw material for inulin production. In this study, inulin was produced from I. helenium roots with a low-cost and easy production method, and it was determined that this inulin was an effective carrier in the synbiotic encapsulation of L. rhamnosus. This inulin exhibits superior prebiotic activity and encapsulation efficiency compared to commercial inulins like Orafti GR and HPX and can be easily integrated into industrial production.
菊粉是一种在食品和制药行业中使用的益生元，它可以促进结肠中有益细菌的生长，从而提高人类健康水平。虽然菊粉是从菊苣和朝鲜蓟中商业化生产的，但旋覆花根为菊粉的生产提供了很高的潜力。本研究旨在研究旋覆花根来源的菊粉（菊粉-P）对鼠李糖乳杆菌的益生元活性，以及其生产共生微胶囊的能力，以及在冷冻干燥、体外胃肠道（GI）消化和储存过程中对益生菌活力的影响。首先，将菊粉-P 对鼠李糖乳杆菌活力和短链脂肪酸（SCFA）产生的影响与其他常用的益生元进行了比较。结果表明，菊粉-P 可显著促进鼠李糖乳杆菌的生长和 SCFA 产量，发酵 48 小时和储存 28 天。然后，用菊粉-P 和商业菊粉包埋鼠李糖乳杆菌，比较其在储存和胃肠道中的存活率。菊粉-P 微胶囊在储存过程中的存活率表现更好（4°C 下储存 30 天后为 7.98logCFU/g）。此外，菊粉-P 微胶囊具有耐热性，并能保护鼠李糖乳杆菌免受 GI 条件的影响，因此在大肠模拟后具有较高的存活率（89.52%），这非常有利于提高客户的收益。此外，菊粉-P 微胶囊具有与商业菊粉相似的物理特性。因此，本研究表明，菊粉-P 易于生产、成本低、具有工业应用潜力，可用作鼠李糖乳杆菌共生包埋的良好载体。实际应用：菊粉是一种益生元，可促进人体肠道中有益细菌的活性和生长。虽然商业菊粉目前是从菊苣根和朝鲜蓟中生产的，但旋覆花根是菊粉生产的潜在原料。在本研究中，采用低成本、易生产的方法从旋覆花根中生产菊粉，并确定该菊粉是鼠李糖乳杆菌共生包埋的有效载体。与 Orafti GR 和 HPX 等商业菊粉相比，这种菊粉具有更好的益生元活性和包埋效率，并且可以很容易地整合到工业生产中。
