Hofmann H, Heinz F X, Dippe H, Kunz C
Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Aug;260(1):132-8. doi: 10.1016/s0176-6724(85)80109-7.
Antibodies found in c.s.f. of patients with TBE are usually produced in the CNS itself, although their presence might also be due to a leak in the blood brain barrier. In the case of local antibody production the ratio of the TBE specific IgM or IgG to the total immunoglobulin is relatively high in c.s.f., whereas in case of a leak this ratio is lower and corresponds to that of the serum. Local antibody production in the brain can therefore be assumed, if the ratio is higher in c.s.f. than in serum. It is possible to determine this ratio in an anti-mu or anti-gamma capturing antibody ELISA, provided that the concentration of capturing antibodies represents the limiting factor of the test system. Under these conditions the absorbance values obtained are a direct reflection of the TBE reactive to nonreactive antibody ratio, since they compet for the limited binding sites presented on the solid phase. Higher extinction values of c.s.f. compared to that of serum are therefore an indication for local antibody production in the brain. Using the method described, differences were found in the production of IgM and IgG antibodies against TBE in the CNS from patients with different clinical history.
在蜱传脑炎（TBE）患者的脑脊液（c.s.f.）中发现的抗体通常是在中枢神经系统（CNS）自身产生的，尽管其存在也可能是由于血脑屏障渗漏所致。在局部抗体产生的情况下，脑脊液中TBE特异性IgM或IgG与总免疫球蛋白的比率相对较高，而在发生渗漏的情况下，该比率较低且与血清中的比率相当。因此，如果脑脊液中的比率高于血清中的比率，则可以假定脑内存在局部抗体产生。如果捕获抗体的浓度是检测系统的限制因素，则可以在抗μ或抗γ捕获抗体ELISA中测定该比率。在这些条件下，获得的吸光度值直接反映了TBE反应性抗体与非反应性抗体的比率，因为它们竞争固相上呈现的有限结合位点。因此，与血清相比，脑脊液的吸光度值更高表明脑内有局部抗体产生。使用所述方法，发现具有不同临床病史的患者中枢神经系统中针对TBE的IgM和IgG抗体产生存在差异。
