Chen Julia, Larsson Ludvig, Swarbrick Alexander, Lundeberg Joakim
Cancer Ecosystems Program, Garvan Institute of Medical Research, Sydney, New South Wales, Australia.
School of Clinical Medicine, Faculty of Medicine and Health, University of New South Wales, Sydney, New South Wales, Australia.
Nat Rev Clin Oncol. 2024 Sep;21(9):660-674. doi: 10.1038/s41571-024-00926-7. Epub 2024 Jul 23.
Solid tumours comprise many different cell types organized in spatially structured arrangements, with substantial intratumour and intertumour heterogeneity. Advances in spatial profiling technologies over the past decade hold promise to capture the complexity of these cellular architectures to build a holistic view of the intricate molecular mechanisms that shape the tumour ecosystem. Some of these mechanisms act at the cellular scale and are controlled by cell-autonomous programmes or communication between nearby cells, whereas other mechanisms result from coordinated efforts between large networks of cells and extracellular molecules organized into tissues and organs. In this Review we provide insights into the application of single-cell and spatial profiling tools, with a focus on spatially resolved transcriptomic tools developed to understand the cellular architecture of the tumour microenvironment and identify opportunities to use them to improve clinical management of cancers.
实体瘤由多种不同类型的细胞组成，这些细胞以空间结构化的方式排列，具有显著的肿瘤内和肿瘤间异质性。在过去十年中，空间分析技术的进展有望捕捉这些细胞结构的复杂性，从而构建出对塑造肿瘤生态系统的复杂分子机制的整体认识。其中一些机制在细胞尺度上起作用，由细胞自主程序或邻近细胞之间的通讯控制，而其他机制则源于组织成组织和器官的大量细胞网络与细胞外分子之间的协同作用。在本综述中，我们深入探讨了单细胞和空间分析工具的应用，重点关注为理解肿瘤微环境的细胞结构而开发的空间分辨转录组学工具，并确定利用这些工具改善癌症临床管理的机会。
