College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China.
School of Chemical Sciences, The University of Auckland, Auckland 1142, New Zealand.
Anal Chem. 2024 Aug 6;96(31):12862-12874. doi: 10.1021/acs.analchem.4c02582. Epub 2024 Jul 24.
Surface-enhanced Raman spectroscopy (SERS) detection platforms with high signal-to-noise ratio in the "biological-silent" region (1800-2800 cm) are presently being developed for sensing and imaging applications, overcoming the limitations of traditional SERS studies in the "fingerprint" region. Herein, a series of cyano-programmable Raman reporters (RRs) operating in the "biological-silent" region were designed based on 4-mercaptobenzonitrile derivatives and then embedded in core-shell Au@Ag nanostars using a "bottom-up" strategy to provide SERS enhancement and encapsulation protection. The approach enabled the "one-pot" readout interference-free detection of multiple bioamines (histamine, tyramine, and β-phenethylamine) based on aptamer-driven magnetic-induced technology. Three cyano-encoded SERS tags resulted in separate SERS signals for histamine, tyramine, and β-phenethylamine at 2220, 2251, and 2150 cm, respectively. A target-specific aptamer-complementary DNA competitive binding strategy allowed the formation of microscale core-satellite assemblies between FeO-based magnetic beads and the SERS tags, enabling multiple SERS signals to be observed simultaneously under a 785 nm laser excitation laser. The LODs for detection of the three bioamines were 0.61 × 10, 2.67 × 10, and 1.78 × 10 mg L, respectively. The SERS-encoded platform utilizing programmable reporters provides a fast and sensitive approach for the simultaneous detection of multiple biomarkers, paving the way for routine SERS analyses of multiple analytes in complex matrices.
目前正在开发具有高信噪比的表面增强拉曼光谱（SERS）检测平台，该平台在“生物静默”区域（1800-2800cm）中进行感应和成像应用，克服了传统 SERS 研究在“指纹”区域的局限性。在此，基于 4-巯基苯甲腈衍生物设计了一系列在“生物静默”区域工作的氰基可编程拉曼报告器（RR），然后使用“自下而上”策略将其嵌入核壳 Au@Ag 纳米星中，以提供 SERS 增强和封装保护。该方法使基于适体驱动磁诱导技术的“一锅法”无干扰读出能够对多种生物胺（组氨酸、酪胺和β-苯乙胺）进行检测。三种氰基编码的 SERS 标记物在 2220、2251 和 2150cm 处分别产生组氨酸、酪胺和β-苯乙胺的单独 SERS 信号。靶标特异性适体互补 DNA 竞争结合策略允许基于 FeO 的磁性珠与 SERS 标记物之间形成微尺度核卫星组装体，从而能够在 785nm 激光激发下同时观察到多个 SERS 信号。三种生物胺的检测下限分别为 0.61×10、2.67×10 和 1.78×10mg L。利用可编程报告器的 SERS 编码平台为同时检测多种生物标志物提供了一种快速灵敏的方法，为复杂基质中多种分析物的常规 SERS 分析铺平了道路。
