Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China.
Langmuir. 2024 Aug 6;40(31):16484-16491. doi: 10.1021/acs.langmuir.4c01849. Epub 2024 Jul 24.
The rapid epidemic around the world of coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, proves the need and stimulates efforts to explore efficient diagnostic tests for the sensitive detection of the SARS-CoV-2 virus. An aggregation-induced electrochemiluminescence (AIECL) sensor was developed for the ultrasensitive detection of the SARS-CoV-2 nucleocapsid (N) protein in this work. Tetraphenylethylene doped in zeolite imidazole backbone-90 (TPE-ZIF-90) showed highly efficient aggregation-induced emission (AIE) to endow TPE-ZIF-90 with high ECL intensity. Upon the capture of the SARS-CoV-2 N protein by immune recognition, an alkaline phosphatase (ALP)-modified gold nanoparticle (AuNP)-decorated zinc oxide (ZnO) nanoflower (ALP/Au-ZnO) composite was introduced on the sensing platform, which catalyzed L-ascorbate-2-phosphate trisodium salt (AA2P) to produce PO and ascorbic acid (AA). Based on a multiquenching of the ECL signal strategy, including resonance energy transfer (RET) between TPE-ZIF-90 and Au-ZnO, disassembly of TPE-ZIF-90 triggered by the strong coordination between PO and Zn, and RET between TPE-ZIF-90 and AuNPs produced in situ by the AA reductive reaction, the constructed AIECL sensor achieved highly sensitive detection of the SARS-CoV-2 N protein with a low limit of detection of 0.52 fg/mL. With the merits of high specificity, good stability, and proven application ability, the present RET- and enzyme-triggered multiquenching AIECL sensor may become a powerful tool in the field of SARS-CoV-2 virus diagnosis.
本工作开发了一种聚集诱导电化学发光（AIECL）传感器，用于灵敏检测 SARS-CoV-2 病毒的核衣壳（N）蛋白。在这项工作中，沸石咪唑酯骨架-90（ZIF-90）掺杂的四苯乙烯（TPE-ZIF-90）表现出高效的聚集诱导发射（AIE），赋予 TPE-ZIF-90 高的电化学发光（ECL）强度。在通过免疫识别捕获 SARS-CoV-2 N 蛋白后，将碱性磷酸酶（ALP）修饰的金纳米粒子（AuNP）修饰的氧化锌（ZnO）纳米花（ALP/Au-ZnO）复合物引入传感平台，该复合物催化 L-抗坏血酸-2-磷酸三钠盐（AA2P）产生 PO 和抗坏血酸（AA）。基于 ECL 信号的多重猝灭策略，包括 TPE-ZIF-90 和 Au-ZnO 之间的共振能量转移（RET）、PO 和 Zn 之间的强配位触发的 TPE-ZIF-90 的解组装以及由 AA 还原反应原位产生的 TPE-ZIF-90 和 AuNPs 之间的 RET，构建的 AIECL 传感器实现了对 SARS-CoV-2 N 蛋白的高灵敏检测，检测限低至 0.52 fg/mL。由于具有高特异性、良好的稳定性和已证明的应用能力，本 RET 和酶触发的多重猝灭 AIECL 传感器可能成为 SARS-CoV-2 病毒诊断领域的有力工具。
