Bandarian Fatemeh, Razi Farideh, Rezaei-Tavirani Mostafa, Arjmand Babak, Jahani Sherafat Somayeh, Rostami Nejad Mohammad
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2024 Jul 7;15:e20. doi: 10.34172/jlms.2024.20. eCollection 2024.
Photoaging that is accompanied by gene expression alteration is known as early aging of the skin due to overexposure to natural and/or artificial ultraviolet radiation (UVR). The assessment of gene expression alteration in human primary neonatal dermal fibroblasts depending on recovery time after exposure to solar simulated ultraviolet radiation (ssUVR) is the main aim of this bioinformatic study. Data are extracted from Gene Expression Omnibus (GEO). The pre-evaluation is done via the GEO2R program. The Significant differentially expressed genes (DEGs) were assessed via protein-protein interaction (PPI) network analysis, and the central genes were identified. The central genes were enriched via gene ontology assessment. Among 224 significant DEGs, 20 central genes including TOP2A, MKI67, BRCA1, HELLS, MAD2L1, ANLN, KIF11, MSH2, KRAS, NCAPG, RFC3, PLK4, WDHD1, BLM, CDKN3, KIF15, SMARCA5, and ATAD2 as hub genes and TOP2A, MKI67, BRCA1, ANLN, KRAS, PLK4, SMARCA5, MMP2, and TLR4 as bottleneck genes were determined. Eight central genes were associated with 16 biological terms. In conclusion, significant differences appeared between gene expression conditions of the cells after 1-day and 5-day recovery. Molecular events include the repair and continuation of photodamages. It is possible to introduce drug targets to prevent the progress of induced damages.
因过度暴露于自然和/或人工紫外线辐射（UVR）而伴有基因表达改变的光老化被称为皮肤早衰。本生物信息学研究的主要目的是评估人类原代新生儿真皮成纤维细胞在暴露于模拟太阳紫外线辐射（ssUVR）后，基因表达改变与恢复时间的关系。数据从基因表达综合数据库（GEO）中提取。通过GEO2R程序进行预评估。通过蛋白质-蛋白质相互作用（PPI）网络分析评估显著差异表达基因（DEG），并确定核心基因。通过基因本体评估对核心基因进行富集分析。在224个显著的DEG中，确定了20个核心基因，包括作为枢纽基因的TOP2A、MKI67、BRCA1、HELLS、MAD2L1、ANLN、KIF11、MSH2、KRAS、NCAPG、RFC3、PLK4、WDHD1、BLM、CDKN3、KIF15、SMARCA5和ATAD2，以及作为瓶颈基因的TOP2A、MKI67、BRCA1、ANLN、KRAS、PLK4、SMARCA5、MMP2和TLR4。八个核心基因与16个生物学术语相关。总之，细胞在恢复1天和5天后的基因表达情况存在显著差异。分子事件包括光损伤的修复和持续。有可能引入药物靶点来阻止诱导损伤的进展。
