Çelik Süheyla Pınar, Parilti Damla Nur, Açik Leyla, Yalçin Mehmet Muhittin, Yetkin İlhan, Yunusov Eldeniz
Department of Biology, Faculty of Science, Gazi University, Ankara, Turkiye.
Department of Endocrinology, Faculty of Medicine, Gazi University, Ankara, Turkiye.
Turk J Biol. 2024 Apr 3;48(2):133-141. doi: 10.55730/1300-0152.2688. eCollection 2024.
BACKGROUND/AIM: Type 2 diabetes mellitus (T2DM) is the most common type of diabetes and occurs due to insufficient insulin secretion or inability to use existing insulin and the effects of environmental factors. Although there are many studies on the pathophysiology of T2DM, the mechanisms contributing to the pathogenesis of insulin resistance and pancreatic beta-cell dysfunction have not been completely elucidated. Some adipokines secreted from adipose tissue, which are the primary regulators of insulin resistance, affect immune and inflammatory functions. Altered adipokine profiles have been observed in obesity and T2DM, leading to severe metabolic risks and changes in insulin sensitivity.
This study used quantitative PCR and ELISA techniques to analyze samples from individuals without diabetes (control group) and with T2DM (macrovascular and microvascular complications and without complications) for at least 10 years.
The mRNA expression and protein levels of NAMPT, IL-6, and vaspin genes were determined. While there was no significant difference in NAMPT, IL-6, and vaspin mRNA expression levels between diabetic groups, there was a significant decrease between the patient and control groups (p < 0.001). For serum protein levels, NAMPT protein levels decreased significantly in the uncomplicated group, while IL-6 and vaspin protein levels increased significantly in both microvascular and macrovascular complication groups (p < 0.001).
The correlations between gene expressions, clinical parameters, and protein levels are crucial to understanding the implications of the findings.
背景/目的：2型糖尿病（T2DM）是最常见的糖尿病类型，其发生是由于胰岛素分泌不足或无法利用现有胰岛素以及环境因素的影响。尽管关于T2DM病理生理学的研究众多，但导致胰岛素抵抗和胰腺β细胞功能障碍发病机制尚未完全阐明。脂肪组织分泌的一些脂肪因子是胰岛素抵抗的主要调节因子，影响免疫和炎症功能。在肥胖和T2DM中已观察到脂肪因子谱的改变，导致严重的代谢风险和胰岛素敏感性变化。
本研究使用定量PCR和ELISA技术，对至少患糖尿病10年的无糖尿病个体（对照组）以及患有T2DM（有大血管和微血管并发症及无并发症）的个体样本进行分析。
测定了NAMPT、IL - 6和vaspin基因的mRNA表达及蛋白水平。糖尿病组之间NAMPT、IL - 6和vaspin mRNA表达水平无显著差异，但患者组与对照组之间有显著降低（p < 0.001）。对于血清蛋白水平，无并发症组NAMPT蛋白水平显著降低，而微血管和大血管并发症组IL - 6和vaspin蛋白水平均显著升高（p < 0.001）。
基因表达、临床参数和蛋白水平之间的相关性对于理解研究结果的意义至关重要。
