Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou, 311300, Zhejiang, China.
Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou, 311300, Zhejiang, China.
J Plant Physiol. 2024 Nov;302:154315. doi: 10.1016/j.jplph.2024.154315. Epub 2024 Jul 19.
The significance of hydrogen sulfide (HS) as a crucial gasotransmitter has been shown extensively in plants, and endogenous HS is often modulated to activate HS signaling when plants respond to numerous developmental and environmental cues. Consequently, elucidating the HS physiological concentrations and the HS generation intensity of plants is key to understanding the activation mechanism of HS signaling, which has attracted increasing attention. Currently, a variety of reaction-based methods have been reported for monitoring HS concentration in vivo and in vitro. In this review, we summarize and describe in detail several methods for quantifying and bioimaging endogenous HS in plants systems, mainly the spectrophotometer-dependent methylene blue (MB) method and fluorescence probes, including the reaction mechanisms, design strategies, response principles, and application details. Moreover, we also summarize the advantages and disadvantages of these methods as well as the research scenarios in which they are applicable. We expect that this review will provide some guidelines on the selection of methods for HS sensing and the comprehensive investigations into HS signaling in plants.
硫化氢 (HS) 作为一种重要的气体信号分子，其在植物中的作用已得到广泛证实。当植物对多种发育和环境信号做出响应时，内源性 HS 通常被调节以激活 HS 信号。因此，阐明植物中 HS 的生理浓度和 HS 的产生强度对于理解 HS 信号的激活机制至关重要，这一机制也引起了越来越多的关注。目前，已经报道了多种基于反应的方法来监测体内和体外 HS 的浓度。在这篇综述中，我们总结并详细描述了几种用于定量和生物成像植物系统内源性 HS 的方法，主要包括分光光度计依赖的亚甲基蓝 (MB) 法和荧光探针，包括反应机制、设计策略、响应原理和应用细节。此外，我们还总结了这些方法的优缺点以及适用的研究场景。我们希望这篇综述能够为 HS 传感方法的选择以及植物中 HS 信号的综合研究提供一些指导。
