Department of Veterinary Medicine, School of Veterinary Medicine and Animal Science, Federal University of Goiás, Goiânia, Goiás, Brazil.
Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
Res Vet Sci. 2024 Sep;177:105363. doi: 10.1016/j.rvsc.2024.105363. Epub 2024 Jul 14.
Cutaneous squamous cell carcinoma (cSCC) is a neoplasm type often diagnosed in dogs. However, studies focused on further investigating its molecular biology, mainly biomarkers to help implementing new therapies, remain scare in the literature. Thus, immunostaining and the gene expression of epidermal growth factor receptors (HER1 and HER2) in canine cSCC presenting different cell differentiation degrees were herein assessed. Thirty-two (32) canine cSCC were selected, classified based on to their cell differentiation degree and subjected to immunohistochemical study to assess HER1 and HER2 immunostaining intensity and distribution. In addition, HER1 and HER2 gene expression was investigated through real-time PCR. Membranous and cytoplasmic immunostaining were observed in both markers. HER2 prevailed in poorly differentiated cSCC; there was positive protein expression correlation between both markers. Mean HER1 gene expression was higher in moderately differentiated, whereas mean HER2 gene expression was higher in poorly differentiated cSCC. Moreover, there was gene expression correlation between markers, regardless of cell differentiation degree. Thus, HER2 protein immunostaining and gene expression were higher in poorly differentiated canine cSCC and it enabled understanding that increase observed in this epidermal growth factor receptor is proportional to this neoplasm's cell differentiation degree in canine species. Results in the current study helped better understanding canine cSCC's molecular biology; however, it is relevant studying other markers aiming to investigate signaling pathways.
皮肤鳞状细胞癌 (cSCC) 是一种常在犬中诊断出的肿瘤类型。然而，针对其分子生物学进行进一步研究的研究，主要是针对生物标志物以帮助实施新疗法的研究，在文献中仍然很少见。因此，本文评估了具有不同细胞分化程度的犬 cSCC 中表皮生长因子受体 (HER1 和 HER2) 的免疫染色和基因表达。选择了 32 个犬 cSCC，根据细胞分化程度进行分类，并进行免疫组织化学研究，以评估 HER1 和 HER2 的免疫染色强度和分布。此外，通过实时 PCR 研究了 HER1 和 HER2 基因表达。在这两种标志物中均观察到膜和细胞质免疫染色。HER2 在分化不良的 cSCC 中占优势；两种标志物之间存在阳性蛋白表达相关性。中等分化的犬 cSCC 的平均 HER1 基因表达较高，而低分化的犬 cSCC 的平均 HER2 基因表达较高。此外，无论细胞分化程度如何，两种标志物之间都存在基因表达相关性。因此，在犬 cSCC 中，HER2 蛋白免疫染色和基因表达在分化不良的肿瘤中更高，这使得我们能够理解在这种表皮生长因子受体中观察到的增加与犬种中这种肿瘤的细胞分化程度成正比。本研究的结果有助于更好地了解犬 cSCC 的分子生物学，但研究其他旨在研究信号通路的标志物也很重要。
