Bendele A M, Neal S B, Oberly T J, Thompson C Z, Bewsey B J, Hill L E, Rexroat M A, Carlton W W, Probst G S
Food Chem Toxicol. 1985 Oct;23(10):911-8. doi: 10.1016/0278-6915(85)90107-3.
Ochratoxin A (OA), a nephrotoxic mycotoxin, was evaluated for genotoxic potential in a battery of in vitro and in vivo assays. OA was not mutagenic to Salmonella typhimurium, either with or without metabolic activation, in the plate incorporation (Ames) test at concentrations of 50-600 micrograms OA/plate or in the gradient plate assay at concentrations of 0.1-1000 micrograms OA/ml. No induction of unscheduled DNA synthesis was evident in primary cultures of rat hepatocytes exposed to concentrations of OA ranging from 0.000025 to 500 micrograms/ml. In the mouse lymphoma forward mutation assay, exposure of L5178Y TK+/- mouse lymphoma cells to OA did not increase the numbers of L5178Y TK-/- mutants. There was no significant difference between the numbers of sister-chromatid exchanges in cells from OA-treated Chinese hamsters and those in cells from the negative-control animals.
赭曲霉毒素A（OA）是一种具有肾毒性的霉菌毒素，通过一系列体外和体内试验对其遗传毒性潜力进行了评估。在平板掺入（艾姆斯）试验中，浓度为50 - 600微克OA/平板时，无论有无代谢激活，OA对鼠伤寒沙门氏菌均无致突变性；在梯度平板试验中，浓度为0.1 - 1000微克OA/毫升时，情况亦是如此。在暴露于浓度范围为0.000025至500微克/毫升OA的大鼠肝细胞原代培养物中，未明显诱导出非程序性DNA合成。在小鼠淋巴瘤正向突变试验中，L5178Y TK+/-小鼠淋巴瘤细胞暴露于OA并未增加L5178Y TK-/-突变体的数量。在经OA处理的中国仓鼠细胞和阴性对照动物细胞中，姐妹染色单体交换的数量没有显著差异。
