Department of Clinical Sciences, College of Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia.
National Unified Procurement Company (NUPCO), Riyadh, Saudi Arabia.
Open Vet J. 2024 Jun;14(6):1358-1369. doi: 10.5455/OVJ.2024.v14.i6.4. Epub 2024 Jun 30.
Healing of bum wounds is commonly associated with many complications. Every year various new repair materials are developed and experimentally used for treating burn wounds. Humans with diabetes mellitus usually suffer from chronic wound healing. Vascular, neuropathic, immune function, and biochemical abnormalities each contribute to the altered tissue repair. One underlying factor that accompanies all diabetic ulcerations is poor vascular flow, a circumstance that impedes proper wound healing. Numerous studies have highlighted the importance of adequate vascular sufficiency and vessel proliferation in tissue repair and the lack thereof in diabetic wound healing. Other studies have looked at whether disarrayed capillary remodeling and maturation of vessels might play a role in impaired diabetic wound healing.
This investigation has been planned to report the influence of treatment with a mixture of both the powder of pomegranate peel (PP) accompanied with an autologous bone marrow (BM) on the cure of burn injuries in experimentally induced diabetic rabbits.
Alloxan monohydrate has been applied to create diabetes in 50 rabbits. Then in each rabbit, two deep second-degree burn wounds were experimentally created. The animals were then divided randomly into 5 treatment sections: non-treatment controls (C1), treated with an available commercial powder for wound (C2), treatment with powder of PP, treatment with alone BM, and the final group treated with PP powder with bone marrow (PPBM). The speed of wound closure and the histopathological changes during healing were measured. The levels of the biomarkers of rabbit platelet-derived growth factor AA (PDGF-AA) and rabbit protease-activated receptor 1 (PAR-1) were measured on days 0, 4, 8, and 12.
Wound healing was markedly more rapid in all the treatment groups versus the control non-treated group. Interestingly, a rapid wound cure was significantly observed in the PPBM group versus the other treatment ones. The histological assessment clarified a significant elevation in the fibroblast and collagen scores in the PPBM group versus the other sections. In addition, there were significant increases in the serum levels of the biomarkers PDGF-AA and PAR-1 among groups.
Dependent on the results of current research, it can be concluded that both PP powder with BM PPBM significantly accelerate the healing process of burn wounds in experimentally induced diabetic rabbits.
烧伤创面的愈合通常与许多并发症有关。每年都会开发和实验性地使用各种新的修复材料来治疗烧伤创面。患有糖尿病的人通常患有慢性伤口愈合不良。血管、神经病变、免疫功能和生化异常都导致组织修复的改变。所有糖尿病溃疡都伴有一个潜在的因素，即血流不良，这会阻碍适当的伤口愈合。许多研究强调了适当的血管充足和血管增殖在组织修复中的重要性，以及在糖尿病伤口愈合中缺乏这些因素的重要性。其他研究还探讨了毛细血管重构和血管成熟紊乱是否在糖尿病伤口愈合不良中起作用。
本研究旨在报告同时使用石榴皮粉（PP）和自体骨髓（BM）混合物治疗对实验性诱导的糖尿病兔烧伤创面愈合的影响。
应用一水合阿脲使 50 只兔子产生糖尿病。然后在每只兔子身上，实验性地造成两个深二度烧伤创面。然后，动物被随机分为 5 个治疗组：非治疗对照组（C1）、用市售的创面粉末治疗组（C2）、用 PP 粉治疗组、用单独 BM 治疗组和最后一组用 PP 粉和骨髓治疗组（PPBM）。测量伤口愈合速度和愈合过程中的组织病理学变化。在第 0、4、8 和 12 天测量兔子血小板衍生生长因子 AA（PDGF-AA）和兔子蛋白酶激活受体 1（PAR-1）的生物标志物水平。
与未治疗的对照组相比，所有治疗组的伤口愈合速度都明显加快。有趣的是，与其他治疗组相比，PPBM 组的伤口愈合速度明显更快。组织学评估表明，PPBM 组的成纤维细胞和胶原评分明显升高，与其他组相比。此外，各组 PDGF-AA 和 PAR-1 的血清水平均显著升高。
根据目前的研究结果，可以得出结论，PP 粉与 BM（PPBM）联合使用可显著加速实验性诱导的糖尿病兔烧伤创面的愈合过程。
