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对意大利拉齐奥和托斯卡纳地区绵羊流产主要传染性病因的生物分子调查。

Biomolecular survey on the main infectious causes of abortion in sheep in the Italian regions of Latium and Tuscany.

机构信息

Isitituto Zooprofilattico Sperimentale del Lazio e della Toscana, Rome, Italy.

出版信息

Open Vet J. 2024 Jun;14(6):1447-1452. doi: 10.5455/OVJ.2024.v14.i6.12. Epub 2024 Jun 30.

DOI:10.5455/OVJ.2024.v14.i6.12
PMID:39055754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11268899/
Abstract

BACKGROUND

Ruminants play an important role in economic sustenance in many developing countries. Abortion is one of the most important causes of economic losses in sheep livestock and, for this reason, it is very important to know, at an early stage, which pathogens caused abortion.

AIM

The aim of the study is to obtain data about the distribution of abortifacient pathogens in the Italian regions of Latium and Tuscany, the awareness of the distribution of infectious agents causing abortion could allow the development of an appropriate vaccination and prophylaxis plan, to avoid major economic losses.

METHODS

388 abortions were collected during the 2015-2018 period. Organs, tissues, and swabs were subjected to DNA extraction and then analyzed with commercial q-PCR kits for the detection of the most common abortion pathogens circulating in these geographical areas.

RESULTS

The positivity in 148 abortions was 56% for , 14% for , 16% for spp, 12% for and 2% for . Interesting results were obtained for cases of abortions with co-infection of abortion pathogens.

CONCLUSION

Diagnosing the cause of abortion remains a multifaceted process that may also include non-infectious factors such as deficiencies and toxicities. Further research is needed also to assess the role of low pathogen concentrations and co-infections in the abortions of sheep.

摘要

背景

反刍动物在许多发展中国家的经济维持中起着重要作用。流产是绵羊养殖业经济损失的最重要原因之一,因此,早期了解哪些病原体引起流产非常重要。

目的

本研究的目的是获得关于意大利拉齐奥和托斯卡纳地区致流产病原体分布的数据。对引起流产的传染性病原体分布的认识可以制定适当的疫苗接种和预防计划,以避免重大的经济损失。

方法

在 2015 年至 2018 年期间收集了 388 例流产。对器官、组织和拭子进行 DNA 提取,然后用商业 q-PCR 试剂盒分析,以检测这些地理区域中常见的流产病原体。

结果

148 例流产中阳性率为:56%为 ,14%为 ,16%为 spp,12%为 ,2%为 。对于流产病原体合并感染的病例,获得了有趣的结果。

结论

诊断流产的原因仍然是一个多方面的过程,也可能包括非传染性因素,如缺乏和毒性。还需要进一步研究,以评估低病原体浓度和合并感染在绵羊流产中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71fa/11268899/25b1ad8a59d6/OpenVetJ-14-1447-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71fa/11268899/7ef496f5aeab/OpenVetJ-14-1447-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71fa/11268899/25b1ad8a59d6/OpenVetJ-14-1447-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71fa/11268899/7ef496f5aeab/OpenVetJ-14-1447-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71fa/11268899/25b1ad8a59d6/OpenVetJ-14-1447-g002.jpg

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Infectious abortions in small domestic ruminants in the Iberian Peninsula: Optimization of sampling procedures for molecular diagnostics.伊比利亚半岛小型家养反刍动物的传染性流产:分子诊断采样程序的优化
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Recent advances and public health implications for environmental exposure to Chlamydia abortus: from enzootic to zoonotic disease.
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Direct economic losses of Toxoplasma gondii abortion outbreaks in two Spanish sheep flocks.两西班牙绵羊群中弓形虫流产暴发的直接经济损失。
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