Sadat Hosseini Sama, Hosseini Seyed Hossein, Hajizade Abbas
Department of Veterinary, Faculty of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Chemistry, Nika Pooyesh Industrial Research Institute, Tehran, Iran.
Heliyon. 2024 Jul 2;10(13):e33960. doi: 10.1016/j.heliyon.2024.e33960. eCollection 2024 Jul 15.
The combination polymers or copolymers have new and combined properties and increase the efficiency of the new polymer. Biopolymers are biodegradable and can play the role of biocompatible and biodegradable in composite polymers. Therefore, poly ortho-toluidine was grafted on chitosan (Cs--POT) by chemical and electrochemical polymerization methods. Cs--POT was characterized by FTIR, UV-visible, and H NMR spectroscopy techniques. The thermal behaviors of the copolymer were investigated by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The images of the surface of the copolymer obtained from imaging SEM confirm the successful attachment of POT on chitosan and indicate that the graft polymerization has been successfully performed with both methods. The percentage and efficiency of engraftment were carefully measured and reported. The electrical conductivity of Cs--POT was measured by the four-point method and the conductivity was 9.1 × 10 S/cm. The copolymer's antibacterial property was studied on , , and as a common bacterium in skin wounds. These studies were investigated using the disk diffusion and minimum inhibitory concentration (MIC) methods. In all tested concentrations the polymer could inhibit the growth of and significantly. However, it inhibited the growth of in concentrations above 1 μg. Bacteria are adsorbed on the surface of the polymer by polar-polar and Van Der Waals interactions, where they undergo cell lysis by dopant and electron transfer, and eventually bacterial cell death. Due to its scaffolding properties, this polymer will have a very good use in tissue and bone repair as well as anti-cancer drugs.
组合聚合物或共聚物具有新的综合性能，提高了新型聚合物的效率。生物聚合物是可生物降解的，在复合聚合物中可起到生物相容性和可生物降解的作用。因此，通过化学和电化学聚合方法将聚邻甲苯胺接枝到壳聚糖（Cs--POT）上。采用傅里叶变换红外光谱（FTIR）、紫外可见光谱和核磁共振氢谱（H NMR）技术对Cs--POT进行了表征。通过热重分析（TGA）和差示扫描量热法（DSC）研究了共聚物的热行为。扫描电子显微镜（SEM）成像获得的共聚物表面图像证实了POT成功附着在壳聚糖上，表明两种方法均成功进行了接枝聚合。仔细测量并报告了接枝率和接枝效率。采用四点法测量了Cs--POT的电导率，电导率为9.1×10 S/cm。以皮肤伤口常见细菌、和为对象研究了该共聚物的抗菌性能。这些研究采用纸片扩散法和最低抑菌浓度（MIC）法进行。在所有测试浓度下，该聚合物均可显著抑制和的生长。然而，在浓度高于1μg时，它可抑制的生长。细菌通过极性-极性相互作用和范德华相互作用吸附在聚合物表面，在聚合物表面通过掺杂剂和电子转移发生细胞裂解，最终导致细菌细胞死亡。由于其支架性能，该聚合物在组织和骨修复以及抗癌药物方面将有很好的应用。
