de Jongh-Leuvenink J, Vreede R W, Marcelis J H, de Vos M, Verhoef J
Infect Immun. 1985 Dec;50(3):716-20. doi: 10.1128/iai.50.3.716-720.1985.
Antisera raised against several smooth and rough strains of Escherichia coli and Salmonella typhimurium were tested against lipopolysaccharides (LPS) of homologous and heterologous strains. The LPS were separated by sodium dodecyl sulfate-gel electrophoresis, transferred to nitrocellulose paper, and overlaid with antisera. The results showed that antisera raised against smooth strains reacted with high- as well as low-molecular-weight bands of their corresponding LPS and showed very few cross-reactions. Anti-E. coli J5 antiserum cross-reacted with few strains in the core region. But, anti-S. typhimurium Ra antiserum cross-reacted with many more strains. When these sera were absorbed with either the homologous- or a heterologous-positive strain, reactions were abolished. It appears that reactions of anti-E. coli J5 antiserum and anti-S. typhimurium Ra antiserum with homologous and heterologous strains were not due to the same antibody. This immunoblotting technique proved to be a useful method to distinguish different antibodies in antiserum raised against LPS of gram-negative bacteria.
用针对几种光滑型和粗糙型大肠杆菌及鼠伤寒沙门氏菌菌株制备的抗血清，检测同源和异源菌株的脂多糖（LPS）。通过十二烷基硫酸钠 - 凝胶电泳分离LPS，转移至硝酸纤维素纸上，并用抗血清覆盖。结果显示，针对光滑型菌株制备的抗血清与相应LPS的高分子量和低分子量条带均发生反应，且交叉反应极少。抗大肠杆菌J5抗血清在核心区域与少数菌株发生交叉反应。但是，抗鼠伤寒沙门氏菌Ra抗血清与更多菌株发生交叉反应。当这些血清用同源或异源阳性菌株吸收后，反应消失。似乎抗大肠杆菌J5抗血清和抗鼠伤寒沙门氏菌Ra抗血清与同源和异源菌株的反应并非由相同抗体引起。这种免疫印迹技术被证明是区分针对革兰氏阴性菌LPS制备的抗血清中不同抗体的有用方法。
