Ito J I, Wunderlich A C, Lyons J, Davis C E, Guiney D G, Braude A I
J Infect Dis. 1980 Oct;142(4):532-7. doi: 10.1093/infdis/142.4.532.
An enzyme-linked immunosorbent assay for antibody to the lipopolysaccharides (LPSs) of a rough strain of Escherichia coli (J5) and Neisseria gonorrhoeae was developed. When standard methods of this assay were applied to these LPSs, no antibody was measured. By radiolabeling smooth and rough endotoxins with 51Cr, it was discovered that the rough LPS was not adhering to the polystyrene tubes. When Mg++ was added, however, all endotoxins absorbed to the solid phase. With this assay antiserum to core glycolipid had higher titers against its smooth parent strain than did homologous glycolipid had higher titers against its smooth parent strain that did homologous antiserum, and antisera to gonococci had high homologous but low heterologous titers. Thus, these data demonstrate that bulky O side chains do not hinder the penetration of antibody to core glycolipid and that LPSs from gonococci are heterogeneous but do cross-react.
开发了一种用于检测大肠杆菌粗糙菌株（J5）和淋病奈瑟菌脂多糖（LPS）抗体的酶联免疫吸附测定法。当将该测定法的标准方法应用于这些LPS时，未检测到抗体。通过用51Cr对光滑型和粗糙型内毒素进行放射性标记，发现粗糙型LPS不粘附于聚苯乙烯管。然而，当加入Mg++时，所有内毒素都吸附到固相上。利用该测定法，核心糖脂抗血清对其光滑亲本菌株的效价比同源糖脂抗血清对其光滑亲本菌株的效价更高，并且淋球菌抗血清具有高同源性但低异源性效价。因此，这些数据表明，庞大的O侧链不会阻碍抗体对核心糖脂的渗透，并且淋球菌的LPS是异质的但确实会发生交叉反应。
