Priority Research Centre for Reproductive Science, The University of Newcastle, Callaghan, New South Wales, Australia.
School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, New South Wales, Australia.
Reproduction. 2024 Aug 27;168(4). doi: 10.1530/REP-24-0049. Print 2024 Oct 1.
In many mammals, the lipid platelet-activating factor (PAF) has important functions in female reproduction and fertility. This study shows that PAF is present in the reproductive tissues of mares and is involved in processes related to ovulation and early pregnancy.
Platelet-activating factor (PAF) has been implicated in a number of reproductive processes ranging from ovulation to embryo motility but has not been widely explored in the mare. To identify the presence and examine the role of PAF in the equine periconception processes, targeted mass spectrometry coupled with chromatographic separation was performed on equine follicular fluid (FF), and PAF was quantitatively detected. Subsequently, untargeted high-resolution mass spectrometry-based lipidomic analysis was carried out to quantify PAF in different-sized pre-ovulatory follicles, whereby different molecular species of PAF, PAF (14:0) and PAF (16:1), were both seen to be increasing with follicle diameter. These findings suggest that PAF within FF is increasing as preovulatory follicles approach ovulation. Additionally, immunofluorescence staining identified the PAF receptor in the luminal pericellular, apical, and basal aspect of equine oviductal epithelial cells. Lastly, an equine oviductal epithelial organoid model was generated and showed that the addition of PAF significantly increased the ciliary beat frequency (CBF) (Hz), an action consistent with a role for PAF in embryo migration. It is proposed that the local action of PAF on the ciliated cells of the oviduct propels both the oocyte and the conceptus towards the uterus. In the mare, it appears that PAF is a contributor during the periconception period, potentially being a mediator in the mechanisms of ovulation and in the dialogue of very early pregnancy.
在许多哺乳动物中，脂质血小板激活因子（PAF）在雌性生殖和生育中具有重要功能。本研究表明，PAF 存在于母马的生殖组织中，并且与排卵和早期妊娠相关的过程有关。
血小板激活因子（PAF）已被牵涉到许多生殖过程中，从排卵到胚胎运动，但在母马中尚未广泛探索。为了确定 PAF 的存在并研究其在马的围孕期过程中的作用，对马卵泡液（FF）进行了靶向质谱联用色谱分离，定量检测了 PAF。随后，进行了非靶向高分辨率基于质谱的脂质组学分析，以定量检测不同大小的预排卵卵泡中的 PAF，结果发现 PAF（14:0）和 PAF（16:1）的不同分子种类均随着卵泡直径的增加而增加。这些发现表明，随着预排卵卵泡接近排卵，FF 中的 PAF 增加。此外，免疫荧光染色鉴定了 PAF 受体在马输卵管上皮细胞的腔周、顶和基底方面。最后，生成了马输卵管上皮类器官模型，并显示 PAF 的添加显著增加了纤毛摆动频率（CBF）（Hz），这一作用与 PAF 在胚胎迁移中的作用一致。有人提出，PAF 对输卵管纤毛细胞的局部作用将卵母细胞和胚胎推向子宫。在母马中，PAF 似乎是围孕期的一个贡献者，可能是排卵和早期妊娠对话机制中的一个介质。
