Prado-Fernández María Fernanda, Magdaleno-Madrigal Víctor Manuel, Cabañas-García Emmanuel, Mucio-Ramírez Samuel, Almazán-Alvarado Salvador, Pérez-Molphe-Balch Eugenio, Gómez-Aguirre Yenny Adriana, Sánchez-Jaramillo Edith
Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, Aguascalientes 20131, Aguascalientes, Mexico.
Laboratorio de Neuromodulación Experimental, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz. México Xochimilco No. 101, Col. San Lorenzo Huipulco, Ciudad de México 14370, Mexico.
Curr Issues Mol Biol. 2024 Jul 3;46(7):6885-6902. doi: 10.3390/cimb46070411.
Griseb. is a plant used in traditional herbal medicine to treat inflammation. We analyzed the phenolic content of leaves (EEPs) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and investigated the anti-inflammatory properties of EEPs and its flavonoid fraction (F10) in animal models subjected to acute neuroinflammation induced by bacterial lipopolysaccharide (LPS). Coronal brain sections of C57BL/6JN male mice or Wistar male rats administered with EEPs or F10 before LPS were subjected to in situ hybridization to determine c-fos and CD14 mRNA levels in the hypothalamus or GABA γ2 mRNA levels in the hippocampus. Theta oscillations were recorded every 6 h in the hippocampus of Wistar rats. In total, five flavonoids and eight phenolic acids were identified and quantified in leaves. Either EEPs or F10 crossed the blood-brain barrier (BBB) into the brain and reduced the mRNA expression of c-fos, CD14, and GABA γ2. A decrease in theta oscillation was observed in the hippocampus of the LPS group, while the F10 + LPS group overrode the LPS effect on theta activity. We conclude that the bioactive compounds of reduce the central response to inflammation, allowing the early return of ambulatory activity and well-being of the animal.
灰黄霉素是一种用于传统草药治疗炎症的植物。我们通过液相色谱 - 串联质谱法（LC-MS/MS）分析了叶片的酚类成分（EEPs），并在由细菌脂多糖（LPS）诱导急性神经炎症的动物模型中研究了EEPs及其黄酮类组分（F10）的抗炎特性。在给予LPS之前给予EEPs或F10的C57BL / 6JN雄性小鼠或Wistar雄性大鼠的冠状脑切片进行原位杂交，以确定下丘脑中的c-fos和CD14 mRNA水平或海马中的GABAγ2 mRNA水平。每6小时记录一次Wistar大鼠海马中的θ振荡。总共在叶片中鉴定并定量了五种黄酮类化合物和八种酚酸。 EEPs或F10均可穿过血脑屏障（BBB）进入大脑，并降低c-fos、CD14和GABAγ2的mRNA表达。在LPS组的海马中观察到θ振荡减少，而F10 + LPS组克服了LPS对θ活动的影响。我们得出结论，灰黄霉素的生物活性化合物可降低中枢对炎症的反应，使动物的自主活动和健康状况早日恢复。
