Stauch Kiri Li N, Black Timothy E, Abramson Charles I
Laboratory of Comparative Psychology and Behavioral Biology, Department of Psychology, Oklahoma State University, Stillwater, OK 74078, USA.
Department of Neuroscience & Psychological Sciences, Weber State University, Ogden, UT 84403, USA.
Insects. 2024 Jul 2;15(7):494. doi: 10.3390/insects15070494.
Honey bees are a commonly used species for alcohol research due to their genome being fully sequenced, their behavioral changes following consumption, and their preference for alcohol. The purpose of this article is to provide a preliminary examination of the genetic expression of heat shock protein 70 (HSP70) and big potassium ion channel protein (BKP) in honey bees following the consumption of either 0%, 2.5%, 5%, or 10% ethanol (EtOH) solutions. The foraging behaviors of the bees were observed and recorded through their return and drinking times. There were significant differences in the return and drinking times between some of the groups. The bees in the 10% condition took significantly longer to return compared to the other groups. Additionally, the bees in the 5% group spent significantly more time drinking compared to the bees in the control (0%) group. There were no significant differences in HSP70 or BKP between the different ethanol groups. Cumulatively, these findings suggest that, while bees may exhibit behavioral differences, the differences in gene expression may not be observed at the transcriptional level.
由于蜜蜂的基因组已被完全测序，它们在摄入酒精后的行为变化以及对酒精的偏好，蜜蜂是酒精研究中常用的物种。本文的目的是初步检测蜜蜂在摄入0%、2.5%、5%或10%乙醇（EtOH）溶液后热休克蛋白70（HSP70）和大钾离子通道蛋白（BKP）的基因表达。通过蜜蜂返回和饮水的时间来观察和记录它们的觅食行为。一些组之间在返回和饮水时间上存在显著差异。与其他组相比，处于10%乙醇条件下的蜜蜂返回所需的时间显著更长。此外，与对照组（0%）的蜜蜂相比，5%组的蜜蜂饮水时间显著更长。不同乙醇组之间的HSP70或BKP没有显著差异。总的来说，这些发现表明，虽然蜜蜂可能表现出行为差异，但在转录水平上可能观察不到基因表达的差异。
