Guagnini Benedetta, Medagli Barbara, Zumbo Bianca, Cannillo Valeria, Turco Gianluca, Porrelli Davide, Bellucci Devis
Department of Medicine, Surgery and Health Sciences, University of Trieste, Piazza dell'Ospitale 1, 34129 Trieste, Italy.
Department of Engineering "Enzo Ferrari", University of Modena and Reggio Emilia, Via P. Vivarelli 10, 41125 Modena, Italy.
J Funct Biomater. 2024 Jul 2;15(7):183. doi: 10.3390/jfb15070183.
In bone regeneration, combining natural polymer-based scaffolds with Bioactive Glasses (BGs) is an attractive strategy to improve the mechanical properties of the structure, as well as its bioactivity and regenerative potential. Methods: For this purpose, a well-studied alginate/hydroxyapatite (Alg/HAp) porous scaffold was enhanced with an experimental bioglass (BGMS10), characterized by a high crystallization temperature and containing therapeutic ions such as strontium and magnesium. This resulted in an improved biological response compared to 45S5 Bioglass, the "gold" standard among BGs. Porous composite scaffolds were fabricated by freeze-drying technique and characterized by scanning electron microscopy and microanalysis, infrared spectroscopy, and microcomputed tomography. The mechanical properties and cytocompatibility of the new scaffold composition were also evaluated. The addition of bioglass to the Alg/HAp network resulted in a slightly lower porosity. However, despite the change in pore size, the MG-63 cells were able to better adhere and proliferate when cultured for one week on a BG scaffold compared to the control Alg/HAp scaffolds. Thus, our findings indicate that the combination of bioactive glass BGMS10 does not affect the structural and physicochemical properties of the Alg/HAp scaffold and confers bioactive properties to the structures, making the Alg/HAp-BGMS10 scaffold a promising candidate for future application in bone tissue regeneration.
在骨再生中，将天然聚合物基支架与生物活性玻璃（BGs）相结合是一种有吸引力的策略，可改善结构的机械性能及其生物活性和再生潜力。方法：为此，用一种实验性生物玻璃（BGMS10）对一种经过充分研究的海藻酸盐/羟基磷灰石（Alg/HAp）多孔支架进行增强，该生物玻璃的特征是具有高结晶温度且含有锶和镁等治疗性离子。与BGs中的“黄金”标准45S5生物玻璃相比，这导致了更好的生物学反应。通过冷冻干燥技术制备多孔复合支架，并通过扫描电子显微镜和微分析、红外光谱以及微型计算机断层扫描对其进行表征。还评估了新支架组合物的机械性能和细胞相容性。向Alg/HAp网络中添加生物玻璃导致孔隙率略有降低。然而，尽管孔径发生了变化，但与对照Alg/HAp支架相比，MG-63细胞在BG支架上培养一周时能够更好地粘附和增殖。因此，我们的研究结果表明，生物活性玻璃BGMS10的组合不会影响Alg/HAp支架的结构和物理化学性质，并赋予结构生物活性，使Alg/HAp-BGMS10支架成为未来骨组织再生应用的有前途的候选者。
