Raveau Robin, Ilbert Chloé, Héloir Marie-Claire, Palavioux Karine, Pébarthé-Courrouilh Anthony, Marzari Tania, Durand Solène, Valls-Fonayet Josep, Cluzet Stéphanie, Adrian Marielle, Fermaud Marc
National Research Institute for Agriculture, Food and the Environment (INRAE), Institute of Vine and Wine Sciences (ISVV), UMR Santé Agroécologie du VignoblE (SAVE), 71 Avenue E. Bourlaux, CS 20032, 33882 Villenave d'Ornon, France.
Agroécologie, National Research Institute for Agriculture, Food and the Environment (INRAE), Institut Agro Dijon, Univ. Bourgogne, 21000 Dijon, France.
J Fungi (Basel). 2024 Jul 9;10(7):471. doi: 10.3390/jof10070471.
Black rot () and downy mildew () are two major grapevine diseases against which the development of efficient biocontrol solutions is required in a context of sustainable viticulture. This study aimed at evaluating and comparing the efficacy and modes of action of bacterial culture supernatants from Buz14 and S38. Both biocontrol agents (BCA) were previously demonstrated as highly effective against in grapevines. In semi-controlled conditions, both supernatants provided significant protection against black rot and downy mildew. They exhibited antibiosis against the pathogens by significantly decreasing mycelial growth, but also the release and motility of zoospores. They also significantly induced grapevine defences, as stilbene production. The LB medium, used for the bacterial cultures, also showed partial effects against both pathogens and induced plant defences. This is discussed in terms of choice of experimental controls when studying the biological activity of BCA supernatants. Thus, we identified two bacterial culture supernatants as new potential biocontrol products exhibiting multi-spectrum antagonist activity against different grapevine key pathogens and having a dual mode of action.
黑腐病（）和霜霉病（）是葡萄的两种主要病害，在可持续葡萄栽培背景下，需要开发有效的生物防治解决方案。本研究旨在评估和比较来自Buz14和S38的细菌培养上清液的功效和作用方式。这两种生物防治剂（BCA）先前已被证明对葡萄中的具有高效性。在半控制条件下，两种上清液都对黑腐病和霜霉病提供了显著的保护作用。它们通过显著降低病原菌的菌丝生长以及游动孢子的释放和游动性，表现出对病原菌的抗生作用。它们还显著诱导了葡萄的防御反应，如芪类化合物的产生。用于细菌培养的LB培养基对两种病原菌也显示出部分作用，并诱导了植物防御反应。在研究BCA上清液的生物活性时，这在实验对照的选择方面进行了讨论。因此，我们鉴定出两种细菌培养上清液作为新的潜在生物防治产品，它们对不同的葡萄关键病原菌表现出多光谱拮抗活性，并具有双重作用方式。
