Zeng Qingchao, Xie Jianbo, Li Yan, Gao Tantan, Zhang Xun, Wang Qi
Department of Plant Pathology, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China.
Front Genet. 2021 Sep 27;12:729603. doi: 10.3389/fgene.2021.729603. eCollection 2021.
has been extensively studied for agricultural application as a biocontrol agent. GLB197, an endophytic bacterium isolated from grape leaves, exhibits distinctive inhibition to grape downy mildew based on unknown mechanisms. To determine the genetic traits involved in the mechanism of biocontrol and host-interaction traits, the genome sequence of GLB197 was obtained and further analyzed. The genome of GLB197 consisted of one plasmid and a 3,733,835-bp circular chromosome with 41.56% G + C content, containing 3,770 protein-coding genes. Phylogenetic analysis of 17 strains using the concatenated 1,226 single-copy core genes divided into different clusters was conducted. In addition, average nucleotide identity (ANI) values indicate that the current taxonomy of some group strains is incorrect. Comparative analysis of GLB197 proteins with other strains identified 3,157 core genes. Furthermore, we found that the pan-genome of is open. The genome of GLB197 contains one nonribosomal peptide synthetase (NRPS) gene cluster which was annotated as lichenysin. Interestingly, the cluster in has two more genes than other strains ( and ). The two genes were probably obtained horizontal gene transfer (HGT) during the evolutionary process from . Taken together, these observations enable the future application of GLB197 as a biocontrol agent for control of grape downy mildew and promote our understanding of the beneficial interactions between GLB197 and plants.
作为一种生物防治剂，其在农业应用方面已得到广泛研究。GLB197是一种从葡萄叶中分离出的内生细菌，基于未知机制对葡萄霜霉病表现出独特的抑制作用。为了确定参与生物防治机制和宿主相互作用特性的遗传特征，获取并进一步分析了GLB197的基因组序列。GLB197的基因组由一个质粒和一条3733835 bp的环状染色体组成，G + C含量为41.56%，包含3770个蛋白质编码基因。利用1226个串联的单拷贝核心基因对17个菌株进行了系统发育分析，这些菌株被分为不同的簇。此外，平均核苷酸同一性（ANI）值表明一些组菌株目前的分类是不正确的。将GLB197的蛋白质与其他菌株进行比较分析，鉴定出3157个核心基因。此外，我们发现其泛基因组是开放的。GLB197的基因组包含一个非核糖体肽合成酶（NRPS）基因簇，该基因簇被注释为地衣ysin。有趣的是，该簇比其他菌株（和）多两个基因。这两个基因可能是在进化过程中通过水平基因转移（HGT）从获得的。综上所述，这些观察结果有助于GLB197未来作为控制葡萄霜霉病的生物防治剂的应用，并增进我们对GLB197与植物之间有益相互作用的理解。
