Shevtsov Alexandr, Izbanova Uinkul, Amirgazin Asylulan, Kairzhanova Alma, Dauletov Ayan, Kiyan Vladimir, Vergnaud Gilles
National Center for Biotechnology, Astana 010000, Kazakhstan.
Aikimbayev's National Scientific Center for Especially Dangerous Infections, Almaty 050000, Kazakhstan.
Pathogens. 2024 Jul 12;13(7):581. doi: 10.3390/pathogens13070581.
Tularemia is an acute febrile disease caused by the Gram-negative bacillus . Based on genetic and phenotypic characteristics, three subspecies are distinguished: , , and . subsp. remains the least studied subspecies. Over the past decade, new foci of distribution of subsp. have been discovered in Russia (Siberia), expanding the possible distribution area by thousands of kilometers. This article provides whole genome single nucleotide polymorphism (wgSNP) and polymorphic tandem repeats (MLVA) analyses of 28 strains isolated between 1965 and 2004 in Kazakhstan. Despite high genetic homogeneity, MLVA with eleven loci (MLVA11) demonstrates a high discriminatory ability (diversity index, 0.9497). The topological structure of the trees based on wgSNP and MLVA is not comparable; however, clustering remains congruent for most outbreaks, with the exception of two strains from one outbreak that are identical in terms of wgSNP but differ at three tandem repeat loci. Based on wgSNP, the strains are assigned to one of the three currently known sublineages, lineage M.I, together with other historical strains maintained in collections in Russia and Sweden. wgSNP shows limited previously unknown genetic diversity, with the M.I lineage size being only 118 SNPs. The wgSNP genotype is not strongly correlated with year and place of isolation.
兔热病是一种由革兰氏阴性杆菌引起的急性发热性疾病。根据遗传和表型特征，可区分出三个亚种：、和。亚种仍然是研究最少的亚种。在过去十年中，在俄罗斯（西伯利亚）发现了亚种的新分布点，使可能的分布区域扩大了数千公里。本文提供了对1965年至2004年期间在哈萨克斯坦分离出的28株菌株的全基因组单核苷酸多态性（wgSNP）和多态串联重复序列（MLVA）分析。尽管遗传同质性较高，但具有11个位点的MLVA（MLVA11）显示出较高的鉴别能力（多样性指数，0.9497）。基于wgSNP和MLVA构建的树的拓扑结构不可比；然而，除了一次暴发中的两株菌株在wgSNP方面相同但在三个串联重复位点上不同外，大多数暴发的聚类结果仍然一致。基于wgSNP，这些菌株被归入目前已知的三个亚种系之一，即M.I系，与俄罗斯和瑞典保藏的其他历史菌株在一起。wgSNP显示出有限的先前未知的遗传多样性，M.I系的大小仅为118个单核苷酸多态性。wgSNP基因型与分离年份和地点没有强烈的相关性。
