College of Engineering, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, China.
Academy of National Food and Strategic Reserves Administration, Beijing 100037, China.
Toxins (Basel). 2024 Jul 4;16(7):305. doi: 10.3390/toxins16070305.
The use of microorganisms to manage aflatoxin contamination is a gentle and effective approach. The aim of this study was to test the removal of AFB from AFB-contaminated peanut meal by a strain of AF01 screened by the authors and to optimize the conditions of the biocontrol. A regression model with the removal ratio of AFB as the response value was established by means of single-factor and response surface experiments. It was determined that the optimal conditions for the removal of AFB from peanut meal by AF01 were 75 h at 29 °C under the natural pH, with an inoculum of 5.5%; the removal ratio of AFB reached 69.31%. The results of simulating solid-state fermentation in production using shallow pans and fermentation bags showed that the removal ratio of AFB was 68.85% and 70.31% in the scaled-up experiments, respectively. This indicated that AF01 had strong adaptability to the environment with facultative anaerobic fermentation detoxification ability. The removal ratio of AFB showed a positive correlation with the growth of AF01, and there were no significant changes in the appearance and quality of the peanut meal after fermentation. This indicated that AF01 had the potential to be used in practical production.
利用微生物来管理黄曲霉毒素污染是一种温和而有效的方法。本研究旨在测试作者筛选的 AF01 菌株对受黄曲霉毒素污染的花生粕中 AFB 的去除效果，并优化其生物防治条件。通过单因素和响应面实验，建立了以 AFB 去除率为响应值的回归模型。结果表明，AF01 去除花生粕中 AFB 的最佳条件为：自然 pH 值下 29°C 培养 75 h，接种量 5.5%；AFB 的去除率达到 69.31%。在浅盘和发酵袋中进行固态发酵模拟生产的结果表明，在扩大规模的实验中，AFB 的去除率分别为 68.85%和 70.31%。这表明 AF01 对兼性厌氧发酵解毒能力的环境具有很强的适应性。AFB 的去除率与 AF01 的生长呈正相关，发酵后花生粕的外观和质量没有明显变化。这表明 AF01 具有在实际生产中应用的潜力。
