Yang Xiaona, Zhang Qi, Chen Zhi-Yuan, Liu Hongxia, Li Peiwu
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, P. R. China.
Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, P. R. China.
PLoS One. 2017 Jun 22;12(6):e0178810. doi: 10.1371/journal.pone.0178810. eCollection 2017.
Pseudomonas fluorescens strain 3JW1, which has a broad-spectrum antimicrobial activity, was studied to investigate whether it affects the amounts of aflatoxin B1 (AFB1) produced by Aspergillus flavus. It was found that the bacterium reduced the amounts of AFB1 in potato dextrose broth (PDB) and peanut medium by 97.8% and 99.4%, respectively. It also reduced AFB1 by ~183 μg/kg (55.8%) when applied onto peanut kernels. This strain reduced AFB1 via three mechanisms. First, it significantly inhibited A. flavus growth; second, our data showed that strain 3JW1 inhibits aflatoxin biosynthesis by A. flavus; and third, P. fluorescens strain 3JW1 is capable of degrading AFB1 at a rate as high as 88.3% in 96 hours. This is the first report demonstrating that Pseudomonas fluorescens can reduce toxin contamination caused by A. flavus on peanut kernels. Our findings indicate that P. fluorescens strain 3JW1 had multiple effects including reducing A. flavus infection and aflatoxin contamination. And the results also highlight the potential applications of the strain 3JW1 for the biological control of aflatoxin contamination in peanuts and other susceptible crops.
荧光假单胞菌3JW1菌株具有广谱抗菌活性，对其进行了研究以调查它是否会影响黄曲霉产生的黄曲霉毒素B1（AFB1）的量。结果发现，该细菌使马铃薯葡萄糖肉汤（PDB）和花生培养基中的AFB1量分别减少了97.8%和99.4%。当将其应用于花生仁时，它还使AFB1减少了约183μg/kg（55.8%）。该菌株通过三种机制减少AFB1。首先，它显著抑制黄曲霉的生长；其次，我们的数据表明3JW1菌株抑制黄曲霉的黄曲霉毒素生物合成；第三，荧光假单胞菌3JW1菌株能够在96小时内以高达88.3%的速率降解AFB1。这是第一份证明荧光假单胞菌可减少花生仁上由黄曲霉引起的毒素污染的报告。我们的研究结果表明，荧光假单胞菌3JW1菌株具有多种作用，包括减少黄曲霉感染和黄曲霉毒素污染。结果还突出了3JW1菌株在花生和其他易感作物黄曲霉毒素污染生物防治方面的潜在应用。
