Li Xuenan, Li Yahui, Dai Xilin
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai 201306, China; National Experimental Teaching Demonstration Centre for Aquatic Sciences, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai 201306, China; National Experimental Teaching Demonstration Centre for Aquatic Sciences, Shanghai Ocean University, Shanghai 201306, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2024 Dec;52:101298. doi: 10.1016/j.cbd.2024.101298. Epub 2024 Jul 23.
Macrobrachium rosenbergii is an economically important crustacean in many parts of the world, but in recent years, growth retardation has become an increasingly serious issue. While the underlying causes remain unclear, this has inevitably impacted on aquaculture and production outputs. In this study, gill, hepatopancreas, and muscle tissue samples from M. rosenbergii, with distinct growth differences, underwent transcriptome sequencing and bioinformatics analyses using high-throughput sequencing. In total, 59,796 unigenes were annotated. Differential expression analyses showed that the most differentially expressed genes (DEGs) were screened in gill tissue (1790 DEGs). In muscle and hepatopancreas tissues, 696 and 598 DEGs were screened, respectively. These DEGs were annotated to Kyoto Encyclopedia of Genes and Genomes pathways, which identified several significantly enriched pathways related to growth metabolism, such as PI3K-AKT, glycolysis/gluconeogenesis, and starch and sucrose metabolism. These results suggest that low growth metabolism levels may be one cause of M. rosenbergii growth retardation. Our data provide support for further investigations into the causes and molecular mechanisms underpinning growth retardation in M. rosenbergii.
罗氏沼虾是世界许多地区具有重要经济价值的甲壳类动物，但近年来，生长迟缓已成为一个日益严重的问题。虽然其根本原因尚不清楚，但这不可避免地影响了水产养殖和产量。在本研究中，对生长差异明显的罗氏沼虾的鳃、肝胰腺和肌肉组织样本进行了高通量测序的转录组测序和生物信息学分析。总共注释了59,796个单基因。差异表达分析表明，在鳃组织中筛选出的差异表达基因（DEG）最多（1790个DEG）。在肌肉和肝胰腺组织中，分别筛选出696个和598个DEG。这些DEG被注释到京都基因与基因组百科全书途径，确定了几个与生长代谢相关的显著富集途径，如PI3K-AKT、糖酵解/糖异生以及淀粉和蔗糖代谢。这些结果表明，低生长代谢水平可能是罗氏沼虾生长迟缓的一个原因。我们的数据为进一步研究罗氏沼虾生长迟缓的原因和分子机制提供了支持。
