College of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.
J Hazard Mater. 2024 Sep 15;477:135296. doi: 10.1016/j.jhazmat.2024.135296. Epub 2024 Jul 22.
A lateral flow immunoassay strip (LFIAS) is one of the most frequently rapid test technologies for carbofuran (CAR). Nevertheless, the LFIAS has a poor quantitative capability and low sensitivity. And, it also requires often complex sample handling steps, making testing time longer. In this study, FeO nanoparticles were successively modified with MIL-100(Fe)-based metal-organic framework (MOF) and chloroplatinic acid hexahydrate to obtain a core-shell complex of FeO-MOF-Pt. The complex had a peroxidase-mimicking activity catalytic function that enabled signal amplification and sensitivity enhancement. Upon coupling with carbofuran monoclonal antibody (CAR-mAb), the magnetic separation properties of the probe enabled target-specific enrichment. The LFIAS based on FeO-MOF-Pt nanocomposites could detect CAR in the range of 0.25-50 ng mL with a limit of detection (LOD) of 0.15 ng mL, enabling colorimetric and catalytic analysis. In addition, the method showed high specificity and stability for detecting CAR in various vegetables, and recovery rates of the spiked samples were 91.40%-102.40%. In conclusion, this study provided one-stop detection of "target enrichment-visual inspection". While lowering the LOD, it reduced the detection time and improved the detection efficiency. The multifunctional FeO-MOF-Pt nanocomposite provides an idea for the construction of novel multifunctional probes to improve the detection performance of conventional LFIAS.
侧向流免疫分析条（LFIAS）是检测克百威（CAR）最常用的快速检测技术之一。然而，LFIAS 的定量能力较差，灵敏度较低。此外，它通常需要复杂的样品处理步骤，导致测试时间延长。在本研究中，FeO 纳米粒子被连续修饰以得到基于 MIL-100(Fe)的金属有机骨架（MOF）和氯铂酸六水合物的核壳复合物 FeO-MOF-Pt。该复合物具有过氧化物酶模拟活性的催化功能，可实现信号放大和灵敏度增强。与克百威单克隆抗体（CAR-mAb）偶联后，探针的磁分离性能实现了对靶标的特异性富集。基于 FeO-MOF-Pt 纳米复合材料的 LFIAS 可以在 0.25-50 ng mL 的范围内检测 CAR，检测限（LOD）为 0.15 ng mL，可进行比色和催化分析。此外，该方法在各种蔬菜中对 CAR 的检测表现出高特异性和稳定性，加标样品的回收率为 91.40%-102.40%。总之，本研究提供了“目标富集-目视检查”的一站式检测。在降低 LOD 的同时，减少了检测时间，提高了检测效率。多功能 FeO-MOF-Pt 纳米复合材料为构建新型多功能探针以提高传统 LFIAS 的检测性能提供了思路。
