Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 967/97, 602 00, Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic; Institute of Laboratory Research on Geomaterials, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská Dolina, Ilkovičova 6, 842 15, Bratislava, Slovakia.
Anal Biochem. 2024 Nov;694:115630. doi: 10.1016/j.ab.2024.115630. Epub 2024 Jul 24.
In this work, we demonstrate the use of capillary electrophoresis and inductively coupled plasma mass spectrometry, as competitive methods primarily for ion chromatography, to determine changes in the concentration of small inorganic ions in the Nostoc sp. culture medium. Although macronutrients were analyzed by capillary electrophoresis with conductivity detection, micronutrients were analyzed by inductively coupled plasma mass spectrometry. The different light settings (light intensity and spectral composition) had a visible effect on the culture growth and depletion of calcium, magnesium, and phosphate ions, and iron and manganese elements when comparing the behavior under red or violet light with that under blue light.
在这项工作中,我们展示了使用毛细管电泳和电感耦合等离子体质谱作为竞争方法,主要用于离子色谱法,来测定念珠藻培养物中小无机离子浓度的变化。虽然使用毛细管电泳和电导检测分析了大量营养素,但使用电感耦合等离子体质谱分析了微量元素。不同的光照条件(光强度和光谱组成)对培养物的生长以及钙、镁和磷酸盐离子以及铁和锰元素的消耗有明显的影响,当比较红光或紫光下的行为与蓝光下的行为时,这种影响更为显著。