Mussin Javier, Giusiano Gustavo
Instituto de Medicina Regional, Universidad Nacional del Nordeste, Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Resistencia 3500, Argentina.
Antibiotics (Basel). 2024 Jul 20;13(7):674. doi: 10.3390/antibiotics13070674.
In response to the steady increase in antimicrobial-resistant strains, the World Health Organisation has emphasised the need to investigate new antimicrobial agents and alternative therapies that improve the spectrum of activity and reduce the dose required, thus improving safety. This study focused on the characterisation of essential oil and green-synthesis silver nanoparticles (AgNP), evaluating their cytotoxicity in human cells, antimicrobial activity and synergistic effect against pathogens causing skin infections. The main components of the essential oil were germacrene A (24.07%), γ-cadinene (21.47%) and trans-caryophyllene (14.97%). Spherical AgNP with a diameter of 15 ± 3 nm were synthesised. The essential oil showed antimicrobial activity against dermatophytes and , while AgNP were found to be active against bacteria, yeasts and dermatophytes. Both compounds were found to be primarily non-cytotoxic at the concentrations required to inhibit microbial growth. Furthermore, the combined use of essential oil and AgNP showed a synergistic antimicrobial effect against dermatophytes and . In conclusion, the results suggest that the combined use of bioactive compounds from natural sources, such as essential oil and biogenic AgNP, has the potential to improve antimicrobial efficacy against specific skin pathogens, particularly , and .
为应对抗菌耐药菌株的不断增加，世界卫生组织强调有必要研究新型抗菌剂和替代疗法，以扩大活性谱并降低所需剂量，从而提高安全性。本研究聚焦于精油和绿色合成银纳米颗粒（AgNP）的特性，评估它们在人类细胞中的细胞毒性、抗菌活性以及对引起皮肤感染的病原体的协同作用。精油的主要成分是杜松烯A（24.07%）、γ-杜松烯（21.47%）和反式石竹烯（14.97%）。合成了直径为15±3纳米的球形AgNP。精油对皮肤癣菌显示出抗菌活性，而AgNP对细菌、酵母和皮肤癣菌有活性。在抑制微生物生长所需的浓度下，两种化合物均被发现基本无细胞毒性。此外，精油和AgNP的联合使用对皮肤癣菌显示出协同抗菌作用。总之，结果表明，天然来源的生物活性化合物如精油和生物源AgNP的联合使用有可能提高对特定皮肤病原体的抗菌效果，特别是对[此处原文缺失具体病原体名称]、[此处原文缺失具体病原体名称]和[此处原文缺失具体病原体名称]。
