Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology, Hechi University, Hechi 546300, China.
Guangxi Collaborative Innovation Center of Modern Sericulture Silk, School of Chemistry and Bioengineering, Hechi University, Hechi 546300, China.
Genes (Basel). 2024 Jun 28;15(7):853. doi: 10.3390/genes15070853.
Cytochrome P450 (CYP) is a crucial oxidoreductase enzyme that plays a significant role in plant defense mechanisms. In this study, a specific cytochrome P450 gene () was discovered in mulberry (). Bioinformatic analysis and expression pattern analysis were conducted to elucidate the involvement of in combating infection. After the infection of , there was a notable increase in the expression of . is overexpressed in Arabidopsis and mulberry and strongly reacts to . The overexpression of the gene in Arabidopsis and mulberry led to a substantial enhancement in resistance against , elevated catalase (CAT) activity, increased proline content, and reduced malondialdehyde (MDA) levels. At the same time, HO and O levels in transgenic Arabidopsis were decreased, which reduced the damage of ROS accumulation to plants. Furthermore, our research indicates the potential involvement of in resistance through the modulation of other resistance-related genes. These findings establish a crucial foundation for gaining deeper insights into the role of cytochrome P450 in mulberry plants.
细胞色素 P450（CYP）是一种重要的氧化还原酶，在植物防御机制中起着重要作用。在这项研究中，我们在桑树（）中发现了一个特定的细胞色素 P450 基因（）。通过生物信息学分析和表达模式分析，阐明了在抵御（）感染中的作用。在感染后，的表达显著增加。在拟南芥和桑树中过表达，并且对强烈反应。在拟南芥和桑树中过表达基因导致对显著增强，过氧化氢酶（CAT）活性升高，脯氨酸含量增加，丙二醛（MDA）水平降低。同时，转基因拟南芥中的 HO 和 O 水平降低，从而减轻了 ROS 积累对植物的损害。此外，我们的研究表明通过调节其他抗性相关基因，可能参与了抗性。这些发现为深入了解细胞色素 P450 在桑树中的作用奠定了重要基础。
