Center for Eco-Environment Restoration Engineering of Hainan Province, School of Ecology, Hainan University, Haikou 570228, China.
Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Yulin Normal University, Yulin 537000, China.
Genes (Basel). 2024 Jul 10;15(7):900. doi: 10.3390/genes15070900.
(Bryaceae, Bryophyta), a rare medicinal bryophyte, is valued for its cardiovascular therapeutic properties in traditional Chinese medicine. This study presents the first complete chloroplast genome sequence of , including its assembly and annotation. The circular chloroplast genome of is 124,315 bp in length, displaying a typical quadripartite structure with 128 genes: 83 protein-coding genes, 37 tRNAs, and 8 rRNAs. Analyses of codon usage bias, repetitive sequences, and simple sequence repeats (SSRs) revealed an A/U-ending codon preference, 96 repetitive sequences, and 385 SSRs in the chloroplast genome. Nucleotide diversity analysis identified 10 high mutational hotspots. Ka/Ks ratio analysis suggested potential positive selection in , , , and genes. Phylogenetic analysis of whole chloroplast genomes from 38 moss species positioned within Bryales, closely related to . This study augments the chloroplast genomic data for Bryales and provides a foundation for molecular marker development and genetic diversity analyses in medicinal bryophytes.
（Bryaceae，Bryophyta），一种罕见的药用苔藓植物，因其在传统中药中的心血管治疗特性而备受重视。本研究首次提供了 的完整叶绿体基因组序列，包括其组装和注释。 的叶绿体基因组全长 124,315 bp，呈现出典型的四分体结构，包含 128 个基因：83 个蛋白质编码基因、37 个 tRNA 和 8 个 rRNA。对密码子使用偏好性、重复序列和简单重复序列（SSR）的分析表明，叶绿体基因组中存在 A/U 结尾的密码子偏好性、96 个重复序列和 385 个 SSR。核苷酸多样性分析确定了 10 个高突变热点。Ka/Ks 比值分析表明，在 、 、 和 基因中存在潜在的正选择。对来自 38 种苔藓物种的整个叶绿体基因组的系统发育分析将 定位在 Bryales 内，与 密切相关。本研究增加了 Bryales 的叶绿体基因组数据，为药用苔藓植物的分子标记开发和遗传多样性分析提供了基础。
