Guangzhou Landscape Plant Germplasm Resource Nursery, Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510540, China.
Guangzhou Collaborative Innovation Center on Science-Tech of Ecology and Landscape, Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510540, China.
Int J Mol Sci. 2023 Oct 13;24(20):15138. doi: 10.3390/ijms242015138.
is popular in ornamental horticulture for its colorful bracts and excellent adaptability, but the complex genetic relationship among this genus is fuzzy due to limited genomic data. To reveal more genomic resources of , we sequenced and assembled the complete chloroplast (cp) genome sequences of 'Splendens'. The cp genome size was 154,869 bp in length, containing 86 protein-coding genes, 38 tRNAs, and eight rRNAs. Cp genome comparison across 12 species (, , , , , , , , , , , and ) revealed five mutational hotspots. Phylogenetic analysis suggested that published previously and clustered into one subclade as two distinct groups, sister to the subclade of 'Splendens'. We considered the phylogeny relationships between and to be controversial. Based on two hypervariable regions and three common plastid regions, we developed five molecular markers for species identification in and applied them to classify 53 ornamental cultivars. This study provides a valuable genetic resource for breeding and offers effective molecular markers to distinguish the representative ornamental species of .
因其色彩斑斓的苞片和极好的适应性，在观赏园艺中很受欢迎，但由于基因组数据有限，该属植物之间复杂的遗传关系尚不明确。为了揭示更多的基因组资源，我们对‘Splendens’的完整叶绿体（cp）基因组序列进行了测序和组装。cp 基因组大小为 154869bp，包含 86 个蛋白编码基因、38 个 tRNA 和 8 个 rRNA。对 12 个种（,,,,,,,,, 和 ）的 cp 基因组比较发现了五个突变热点。系统发育分析表明，先前发表的 和 聚为一簇，与‘Splendens’的亚群形成两个不同的分支。我们认为 和 之间的系统发育关系存在争议。基于两个高变区和三个常见的质体区，我们开发了用于鉴定 和 种的五个分子标记，并将其应用于对 53 个观赏品种的分类。本研究为 的培育提供了有价值的遗传资源，并提供了有效的分子标记来区分有代表性的观赏物种。
