Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at Landscape Architecture and Arts, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2023 Jul 6;24(13):11167. doi: 10.3390/ijms241311167.
, a genus of perennial herbs from the family Orchidaceae, contains a number of ornamental species. However, there is no information on the chloroplast genomes of , which limits our studies of this genus. In this study, we reported the chloroplast genomes of three species of (, , and 'Semi-alba') and performed comprehensive comparative analysis. These three chloroplast genomes showed a typical quadripartile structure. Their lengths ranged from 147,311 bp to 149,240 bp. Each genome contained 120 unique genes, including 74 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Comparative analysis revealed major differences in sequence divergence in the three chloroplast genomes. In addition, six hypervariable regions were identified (, , , , , ) that can be used as DNA molecular markers. Phylogenetic relationships were determined using the chloroplast genomes of 28 species from 12 genera of Aeridinae. Results suggested that was a clade of Aeridinae that was sister to the clade, with 100% bootstrap support within Aeridinae. The findings of this study provided the foundation for future studies on the phylogenetic analysis of Aeridinae.
杓兰属，兰科杓兰亚科的多年生草本植物，包含了一些观赏种。然而，关于杓兰属的叶绿体基因组信息尚未可知，这限制了我们对该属的研究。在本研究中，我们报道了三种杓兰属植物（, , 'Semi-alba'）的叶绿体基因组，并进行了全面的比较分析。这三个叶绿体基因组呈现出典型的四分体结构。它们的长度范围从 147311bp 到 149240bp。每个基因组包含 120 个独特的基因，包括 74 个蛋白编码基因、38 个 tRNA 基因和 8 个 rRNA 基因。比较分析显示，这三个叶绿体基因组在序列上存在显著差异。此外，还鉴定出了六个高变区（,,,,, ），可作为 DNA 分子标记。利用 Aeridinae 亚科 12 个属的 28 种植物的叶绿体基因组进行了系统发育分析。结果表明，杓兰属是 Aeridinae 中的一个分支，与 分支并列，在 Aeridinae 中具有 100%的自展支持率。本研究的结果为 Aeridinae 的系统发育分析提供了基础。
