Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, No. 16 Donghai Road, West Tuanbo New Town, Jinghai District, Tianjin 301617, China.
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
Int J Mol Sci. 2024 Jul 18;25(14):7883. doi: 10.3390/ijms25147883.
Epinephrine (EP) is a very important chemical transmitter in the transmission of nerve impulses in the central nervous system of mammals. Ascorbic acid (AA) is considered to be the most important extracellular fluid antioxidant and has important antioxidant properties in the cell. In this study, a series of transition metal-polyhistidine-carboxylated multi-wall carbon nanotube nanocomposites were synthesized, and their simultaneous catalytic effects on epinephrine and ascorbic acid were investigated. The results showed that nanocomposites based on iron ions had the highest catalytic activity. The prepared biosensor expressed high selectivity toward EP and AA with LOD values of 0.1 μΜ (AA) and 0.01 μΜ (EP), and sensitivity values of 4.18 μA mM with a range of 0.001-5 mM (AA), 50.98 μA mM with a range of 0.2-100 μM (EP), and 265.75 μA mM with a range of 0.1-1.0 mM (EP). Moreover, it showed good stability, good repeatability and high selectivity in real sample detection. This work is a reference for the design of new electrochemical enzyme-free biosensors and the detection of biomarkers.
肾上腺素（EP）是哺乳动物中枢神经系统中神经冲动传递的一种非常重要的化学递质。抗坏血酸（AA）被认为是最重要的细胞外液抗氧化剂，具有重要的抗氧化特性。在这项研究中，合成了一系列过渡金属-多组氨酸-羧化多壁碳纳米管纳米复合材料，并研究了它们对肾上腺素和抗坏血酸的同时催化作用。结果表明，基于铁离子的纳米复合材料具有最高的催化活性。该制备的生物传感器对 EP 和 AA 表现出高选择性，LOD 值分别为 0.1 μΜ（AA）和 0.01 μΜ（EP），灵敏度值分别为 0.001-5 mM（AA）范围内的 4.18 μA mM、0.2-100 μM（EP）范围内的 50.98 μA mM 和 0.1-1.0 mM（EP）范围内的 265.75 μA mM。此外，它在实际样品检测中表现出良好的稳定性、良好的重现性和高选择性。这项工作为新型电化学无酶生物传感器的设计和生物标志物的检测提供了参考。
