Yang Shih-An, Cheng Po-Hsun, Hsu Yi-Ju, Cheng Shu-Feng, Lin Meng-Hsueh Amanda, Huang Chi-Chang
Product Development & Research Institute, Vedan Biotechnology, Taichung 43351, Taiwan.
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan.
Foods. 2024 Jul 16;13(14):2232. doi: 10.3390/foods13142232.
ChlorichEnergyBoost, a water extract obtained from , has been proposed to enhance physical performance and provide anti-fatigue effects. This study assessed the impact of ChlorichEnergyBoost supplementation on physical performance and its anti-fatigue properties. Twenty-four mice were allocated into four groups: (1) the control group receiving only water,;(2) the 1X group (49.2 mg/kg/day); (3) the 2X group (98.4 g/kg/day); and (4) the 5X group (246 g/kg/day). All groups were orally administered the supplements for four consecutive weeks. The evaluation included grip strength, swimming endurance, an exhaustion test, and serum biochemistry analysis. Additionally, the study examined the bioactive peptides through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) and conducted bacterial reverse mutation and acute oral toxicity tests for safety assessment. The findings indicated that ChlorichEnergyBoost supplementation led to a significant reduction in serum lactate levels by 14.08% to 22.54% and blood urea nitrogen levels by 12.23% to 16.76%, an increase in the lactate clearance rate by 0.28 to 0.35, an enhancement of muscle glycogen storage by 1.10 to 1.44-fold, and hepatic glycogen storage by 1.41 to 1.47-fold. These results demonstrated dose-dependent effects. MALDI-TOF analysis revealed the expression of dihydrolipoamide dehydrogenase and superoxide dismutase. Both the bacterial reverse mutation and acute oral toxicity tests showed no adverse effects.
ChlorichEnergyBoost是一种从[具体来源未给出]中提取的水提取物，已被提议可增强身体机能并提供抗疲劳效果。本研究评估了补充ChlorichEnergyBoost对身体机能及其抗疲劳特性的影响。将24只小鼠分为四组：(1) 仅接受水的对照组；(2) 1X组（49.2毫克/千克/天）；(3) 2X组（98.4克/千克/天）；以及(4) 5X组（246克/千克/天）。所有组连续四周口服补充剂。评估包括握力、游泳耐力、疲劳试验和血清生化分析。此外，该研究通过基质辅助激光解吸/电离质谱（MALDI-TOF MS）检测生物活性肽，并进行细菌回复突变和急性经口毒性试验以进行安全性评估。研究结果表明，补充ChlorichEnergyBoost可使血清乳酸水平显著降低14.08%至22.54%，血尿素氮水平降低12.23%至16.76%，乳酸清除率提高0.28至0.35，肌肉糖原储存增加1.10至1.44倍，肝糖原储存增加1.41至1.47倍。这些结果显示出剂量依赖性效应。MALDI-TOF分析揭示了二氢硫辛酰胺脱氢酶和超氧化物歧化酶的表达。细菌回复突变试验和急性经口毒性试验均未显示出不良反应。
