Lee Ji Yeon, Kang Chang-Ho
MEDIOGEN, Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, Korea.
Microorganisms. 2022 Jan 21;10(2):234. doi: 10.3390/microorganisms10020234.
Antioxidants protect against oxidative stress that can damage proteins, the cellular immune system, and DNA. In recent studies, probiotics have been shown to impart a microbial balance to the gastrointestinal tract, demonstrating significant antioxidant capacity. In this study, the probiotic properties and antioxidant mechanism of probiotics were evaluated in HepG2 cells and in an animal model. The characteristics of MG5125, MG731, and MG741, which were used as lactic acid bacteria in this study, were analyzed. The results revealed the safety and stability of these probiotics in the gastrointestinal tract because they did not cause hemolysis and had excellent intestinal adhesion (75-84%). In HepG2 cells, the three probiotics alleviated HO-induced oxidative stress by mediating lipid peroxidation and glutathione levels and upregulating antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase. In the tBHP-induced mouse model, administration of the three probiotics reduced hepatic aspartate transaminase, alanine transaminase, and lipid peroxidation levels. In conclusion, MG5125, MG731, and MG741 showed considerable antioxidant activity both in vitro and in vivo.
抗氧化剂可抵御氧化应激，氧化应激会损害蛋白质、细胞免疫系统和DNA。在最近的研究中，益生菌已被证明能使胃肠道微生物保持平衡，并具有显著的抗氧化能力。在本研究中，在HepG2细胞和动物模型中评估了益生菌的益生菌特性和抗氧化机制。分析了本研究中用作乳酸菌的MG5125、MG731和MG741的特性。结果显示这些益生菌在胃肠道中具有安全性和稳定性，因为它们不会引起溶血，并且具有出色的肠道黏附性（75-84%）。在HepG2细胞中，这三种益生菌通过介导脂质过氧化和谷胱甘肽水平以及上调抗氧化酶（包括过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶）来减轻过氧化氢诱导的氧化应激。在叔丁基过氧化氢诱导的小鼠模型中，给予这三种益生菌可降低肝脏天冬氨酸转氨酶、丙氨酸转氨酶和脂质过氧化水平。总之，MG5125、MG731和MG741在体外和体内均表现出相当大的抗氧化活性。
