Ponton J, Quindos G, Regulez P, Cisterna R
Mycopathologia. 1985 Oct;92(1):11-8. doi: 10.1007/BF00442653.
Pure mycelial and yeast cultures of Candida albicans were produced in a low sulphate medium. Groups of mice were injected subcutaneously with increasing doses of viable or heat-killed mycelial or yeast cells and the kinetics of delayed-type hypersensitivity (DTH), anti-mycelial and anti-yeast antibodies were studied. Both the dose and the morphological phase of C. albicans showed an influence on the development of the DTH, but the viability is the factor which showed the highest influence on this reaction, since on the one hand mice infected with viable yeast or mycelial cells developed higher DTH levels than mice injected with heat killed cells, and on the other hand this factor seems to play an important role in the kinetics of DTH response. The enzyme-linked immunosorbent assay has been adapted to detect antibodies to yeast and mycelial phase cytoplasmic antigens of C. albicans. In contrast with the DTH reactions, neither dose, morphological phase nor viability played an important role on the antibody titer developed. However, the use of mycelial cytoplasmic antigens seems to be better than the yeasts to detect anti-Candida antibodies over the last days studied.
白色念珠菌的纯菌丝体和酵母培养物在低硫酸盐培养基中产生。给几组小鼠皮下注射递增剂量的活的或热灭活的菌丝体细胞或酵母细胞，并研究迟发型超敏反应（DTH）、抗菌丝体和抗酵母抗体的动力学。白色念珠菌的剂量和形态阶段均对DTH的发展有影响，但活力是对该反应影响最大的因素，因为一方面，感染活酵母或菌丝体细胞的小鼠比注射热灭活细胞的小鼠产生更高水平的DTH，另一方面，该因素似乎在DTH反应的动力学中起重要作用。酶联免疫吸附测定已适用于检测针对白色念珠菌酵母和菌丝体相细胞质抗原的抗体。与DTH反应相反，剂量、形态阶段和活力对所产生的抗体滴度均未起重要作用。然而，在研究的最后几天里，使用菌丝体细胞质抗原似乎比酵母更能检测抗念珠菌抗体。
