Department of Urology, University of Patras, 26504 Patras, Greece.
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National Kapodistrian University of Athens (NKUA), 11527 Athens, Greece.
Medicina (Kaunas). 2024 Jul 8;60(7):1108. doi: 10.3390/medicina60071108.
The presence and contribution of senescent cells in premalignant lesions is well documented, but not in germ cell neoplasia in situ. The purpose of this study is to identify the presence of senescent cells in pre-malignant testicular conditions and in different histological types of testicular cancer. Thirty patients who underwent orchiectomy due to testicular tumors were included. Formalin-fixed paraffin-embedded (FFPE) testicular tissue for each patient was available. Sections from these specimens were examined by immunohistochemical analysis with the following markers: GL13 for cellular senescence, p21 for cell cycle arrest, and Ki67 for cell proliferation. Thirteen (43.3%) suffered from seminoma with a mean total proportion of GCNIS senescence of 20.81 ± 6.81%. In the group of embryonal testicular tumors, nine (30%) patients were included, with an average rate of 6.64 ± 5.42% of senescent cells in GCNIS. One (3.3%) patient suffered from chondrosarcoma in which 7.9% of GL13+ cells were detected in GCNIS. Four (13.4%) patients suffered from teratoma and three (10%) from yolk sac tumors, while GCNIS senescence was detected in a range of 4.43 ± 1.78% and 3.76 ± 1.37%, respectively. Cellular senescence was detected in both germ cell neoplasia in situ and testicular cancer, but was more prevalent within the premalignant lesions.
衰老细胞在癌前病变中的存在和贡献已有充分的文献记载，但在生殖细胞原位肿瘤中则不然。本研究旨在确定衰老细胞在睾丸癌前病变和不同组织学类型的睾丸癌中的存在情况。
纳入了 30 名因睾丸肿瘤而行睾丸切除术的患者。每位患者的福尔马林固定石蜡包埋（FFPE）睾丸组织均可获得。通过免疫组织化学分析检查这些标本，使用以下标志物进行分析：GL13 用于细胞衰老，p21 用于细胞周期停滞，Ki67 用于细胞增殖。
13 名（43.3%）患者患有精原细胞瘤，GCNIS 衰老的总比例平均为 20.81±6.81%。在胚胎性睾丸肿瘤组中，纳入了 9 名（30%）患者，GCNIS 中衰老细胞的平均比例为 6.64±5.42%。1 名（3.3%）患者患有软骨肉瘤，在 GCNIS 中检测到 7.9%的 GL13+细胞。4 名（13.4%）患者患有畸胎瘤，3 名（10%）患者患有卵黄囊肿瘤，而 GCNIS 衰老的范围分别为 4.43±1.78%和 3.76±1.37%。
在生殖细胞原位肿瘤和睾丸癌中均检测到细胞衰老，但在癌前病变中更为常见。
