Rakov Alexey V, Chekanova Tatiana A, Petremgvdlishvili Ketevan, Linnik Svetlana B, Batchaev Khusey Kh, Akimkin Vasiliy G
Laboratory for Natural Focal Infections Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, Russia.
Center of Hygiene and Epidemiology in the Karachay-Cherkess Republic, 369000 Cherkessk, Russia.
Microorganisms. 2024 Jun 25;12(7):1293. doi: 10.3390/microorganisms12071293.
Karachay-Cherkessia is the region in the Russian Federation that has been overlooked in terms of research on the human bacterial pathogens transmitted by ticks. In this study, we investigated the infection status of ticks with , , , , and / associated with natural focal infections in Karachay-Cherkessia. A total amount of 159 ticks from three species across three genera (, , ) removed from humans were collected for analysis. Additionally, we used 53 individual ticks and 40 tick pools from the vegetation of three species (, , and ). Screening of the studied pathogens was performed by using commercial qPCR kits. Sanger sequencing utilizing partial sequences of and genes was employed to identify the species. Our findings revealed the presence of DNA from five species within the spotted fever group , namely , , , , and . Moreover, two distinct genotypes were identified within , , and . Additionally, DNA from s.l., , and was detected in the tested ticks. This study provides valuable insights into the prevalence and the diversity of bacterial pathogens transmitted by ticks in the Karachay-Cherkessia region.
卡拉恰伊－切尔克斯共和国是俄罗斯联邦中一个在蜱传播的人类细菌病原体研究方面被忽视的地区。在本研究中，我们调查了卡拉恰伊－切尔克斯共和国与自然疫源感染相关的蜱感染 、 、 、 以及 / 的状况。从人类身上采集了来自三个属（ 、 、 ）的三个物种的总共159只蜱用于分析。此外，我们还使用了来自三个物种（ 、 、 ）植被中的53只个体蜱和40个蜱虫样本。通过使用商业qPCR试剂盒对所研究的病原体进行筛选。利用 和 基因的部分序列进行桑格测序来鉴定 物种。我们的研究结果揭示了斑点热群 内五个物种的DNA存在，即 、 、 、 以及 。此外，在 、 以及 中鉴定出了两种不同的基因型。此外，在测试的蜱中检测到了狭义 、 以及 的DNA。本研究为卡拉恰伊－切尔克斯共和国地区蜱传播的细菌病原体的流行情况和多样性提供了有价值的见解。
