• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

磷脂生物合成转录调节因子的缺失改变了酿酒酵母中Sec61转运体β亚基Sbh1的翻译后修饰。 （注：原文中“in.”后面应该补充完整信息，这里根据常见情况推测补充了“酿酒酵母”，具体需根据完整原文确定。）

Loss of transcriptional regulator of phospholipid biosynthesis alters post-translational modification of Sec61 translocon beta subunit Sbh1 in .

作者信息

Miller Jacob M, Tragesser-Tiña Mary E, Turk Samantha M, Rubenstein Eric M

机构信息

Department of Biology, Ball State University.

Diabetes, Obesity, and Complications Therapeutic Area, Eli Lilly and Company.

出版信息

MicroPubl Biol. 2024 Jul 12;2024. doi: 10.17912/micropub.biology.001260. eCollection 2024.

DOI:10.17912/micropub.biology.001260
PMID:39071171
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11282434/
Abstract

We recently discovered that disrupting phospholipid biosynthesis by eliminating the Ino2/4 transcriptional regulator impairs endoplasmic reticulum (ER)-associated degradation (ERAD) in , but the mechanism is unclear. Phosphatidylcholine deficiency has been reported to accelerate degradation of Sec61 translocon beta subunit Sbh1 and ERAD cofactor Cue1. Here, we found that, unlike targeted phosphatidylcholine depletion, deletion does not destabilize Sbh1 or Cue1. However, we observed altered electrophoretic mobility of Sbh1 in Δ yeast, consistent with phospholipid-responsive post-translational modification. A better understanding of the molecular consequences of disrupted lipid homeostasis could lead to enhanced treatments for conditions associated with perturbed lipid biosynthesis.

摘要

我们最近发现，通过消除Ino2/4转录调节因子来破坏磷脂生物合成会损害内质网（ER）相关降解（ERAD），但其机制尚不清楚。据报道，磷脂酰胆碱缺乏会加速Sec61转运体β亚基Sbh1和ERAD辅助因子Cue1的降解。在这里，我们发现，与靶向磷脂酰胆碱消耗不同，Ino2/4缺失不会使Sbh1或Cue1不稳定。然而，我们观察到Ino2/4缺失的酵母中Sbh1的电泳迁移率发生了改变，这与磷脂反应性翻译后修饰一致。更好地理解脂质稳态破坏的分子后果可能会增强对与脂质生物合成紊乱相关病症的治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23de/11282434/14d7d798281a/25789430-2024-micropub.biology.001260.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23de/11282434/14d7d798281a/25789430-2024-micropub.biology.001260.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23de/11282434/14d7d798281a/25789430-2024-micropub.biology.001260.jpg

相似文献

1
Loss of transcriptional regulator of phospholipid biosynthesis alters post-translational modification of Sec61 translocon beta subunit Sbh1 in .磷脂生物合成转录调节因子的缺失改变了酿酒酵母中Sec61转运体β亚基Sbh1的翻译后修饰。 （注：原文中“in.”后面应该补充完整信息，这里根据常见情况推测补充了“酿酒酵母”，具体需根据完整原文确定。）
MicroPubl Biol. 2024 Jul 12;2024. doi: 10.17912/micropub.biology.001260. eCollection 2024.
2
Lipid biosynthesis perturbation impairs endoplasmic reticulum-associated degradation.脂质生物合成干扰破坏内质网相关降解。
J Biol Chem. 2023 Aug;299(8):104939. doi: 10.1016/j.jbc.2023.104939. Epub 2023 Jun 17.
3
Sec61 channel subunit Sbh1/Sec61β promotes ER translocation of proteins with suboptimal targeting sequences and is fine-tuned by phosphorylation.Sec61 通道亚基 Sbh1/Sec61β 促进具有次优靶向序列的蛋白质向 ER 的易位，并通过磷酸化进行精细调节。
J Biol Chem. 2023 Mar;299(3):102895. doi: 10.1016/j.jbc.2023.102895. Epub 2023 Jan 11.
4
The ER Protein Translocation Channel Subunit Sbh1 Controls Virulence of Cryptococcus neoformans.内质网蛋白易位通道亚基 Sbh1 控制新型隐球菌的毒力。
mBio. 2023 Feb 28;14(1):e0338422. doi: 10.1128/mbio.03384-22. Epub 2023 Feb 7.
5
Effect of Sec61 interaction with Mpd1 on endoplasmic reticulum-associated degradation.Sec61 与 Mpd1 相互作用对内质网相关降解的影响。
PLoS One. 2019 Jan 25;14(1):e0211180. doi: 10.1371/journal.pone.0211180. eCollection 2019.
6
Global rewiring of lipid metabolism to produce carotenoid by deleting the transcription factor genes ino2/ino4 in Saccharomyces cerevisiae.在酿酒酵母中通过删除转录因子基因 ino2/ino4 来实现脂质代谢的全局重布线以产生类胡萝卜素。
Int J Biol Macromol. 2024 Apr;264(Pt 1):130400. doi: 10.1016/j.ijbiomac.2024.130400. Epub 2024 Feb 25.
7
Influence of gene dosage and autoregulation of the regulatory genes INO2 and INO4 on inositol/choline-repressible gene transcription in the yeast Saccharomyces cerevisiae.基因剂量以及调控基因INO2和INO4的自动调节对酿酒酵母中肌醇/胆碱可抑制基因转录的影响。
Curr Genet. 1997 Jun;31(6):462-8. doi: 10.1007/s002940050231.
8
Dimerization of yeast transcription factors Ino2 and Ino4 is regulated by precursors of phospholipid biosynthesis mediated by Opi1 repressor.酵母转录因子Ino2和Ino4的二聚化受由Opi1阻遏物介导的磷脂生物合成前体的调控。
Curr Genet. 2008 Jul;54(1):35-45. doi: 10.1007/s00294-008-0197-7. Epub 2008 Jun 10.
9
Membrane phospholipid alteration causes chronic ER stress through early degradation of homeostatic ER-resident proteins.膜脂的改变通过早期降解内质网驻留蛋白引起内质网慢性应激。
Sci Rep. 2019 Jun 14;9(1):8637. doi: 10.1038/s41598-019-45020-6.
10
The INO2 and INO4 loci of Saccharomyces cerevisiae are pleiotropic regulatory genes.酿酒酵母的INO2和INO4基因座是多效性调控基因。
Mol Cell Biol. 1984 Nov;4(11):2479-85. doi: 10.1128/mcb.4.11.2479-2485.1984.

引用本文的文献

1
The kinesin Kar3 is required for endoplasmic reticulum-associated degradation.驱动蛋白Kar3是内质网相关降解所必需的。
Mol Biol Cell. 2025 Mar 1;36(3):br9. doi: 10.1091/mbc.E24-10-0437. Epub 2025 Jan 22.

本文引用的文献

1
Lipid biosynthesis perturbation impairs endoplasmic reticulum-associated degradation.脂质生物合成干扰破坏内质网相关降解。
J Biol Chem. 2023 Aug;299(8):104939. doi: 10.1016/j.jbc.2023.104939. Epub 2023 Jun 17.
2
Sec61 channel subunit Sbh1/Sec61β promotes ER translocation of proteins with suboptimal targeting sequences and is fine-tuned by phosphorylation.Sec61 通道亚基 Sbh1/Sec61β 促进具有次优靶向序列的蛋白质向 ER 的易位，并通过磷酸化进行精细调节。
J Biol Chem. 2023 Mar;299(3):102895. doi: 10.1016/j.jbc.2023.102895. Epub 2023 Jan 11.
3
The ERAD system is restricted by elevated ceramides.
内质网相关降解（ERAD）系统受到神经酰胺水平升高的限制。
Sci Adv. 2023 Jan 13;9(2):eadd8579. doi: 10.1126/sciadv.add8579.
4
Saccharomyces genome database update: server architecture, pan-genome nomenclature, and external resources.酿酒酵母基因组数据库更新：服务器架构、泛基因组命名法和外部资源。
Genetics. 2023 May 4;224(1). doi: 10.1093/genetics/iyac191.
5
Phospholipids: Identification and Implication in Muscle Pathophysiology.磷脂：在肌肉病理生理学中的鉴定和意义。
Int J Mol Sci. 2021 Jul 30;22(15):8176. doi: 10.3390/ijms22158176.
6
In-depth and 3-dimensional exploration of the budding yeast phosphoproteome.深入且多维地探索 budding yeast 磷酸化蛋白质组。
EMBO Rep. 2021 Feb 3;22(2):e51121. doi: 10.15252/embr.202051121. Epub 2021 Jan 25.
7
Cross-compartment signal propagation in the mitotic exit network.有丝分裂后期网络中隔室间信号的传播。
Elife. 2021 Jan 22;10:e63645. doi: 10.7554/eLife.63645.
8
Beyond Proteostasis: Lipid Metabolism as a New Player in ER Homeostasis.超越蛋白质稳态：脂质代谢成为内质网稳态的新参与者
Metabolites. 2021 Jan 14;11(1):52. doi: 10.3390/metabo11010052.
9
Overlapping function of Hrd1 and Ste24 in translocon quality control provides robust channel surveillance.Hrd1 和 Ste24 在易位子质量控制中的重叠功能提供了稳健的通道监控。
J Biol Chem. 2020 Nov 20;295(47):16113-16120. doi: 10.1074/jbc.AC120.016191. Epub 2020 Oct 8.
10
Phosphoproteome Response to Dithiothreitol Reveals Unique Shared Features of Stress Responses.磷酸化蛋白质组对二硫苏糖醇的反应揭示了应激反应独特的共同特征。
J Proteome Res. 2020 Aug 7;19(8):3405-3417. doi: 10.1021/acs.jproteome.0c00253. Epub 2020 Jul 13.