Jun Liang, Wang Zhonghua, Wang Shouhong, Liao Xiaolong, Qin Tiehe, Guo Weixin
Department of Intensive Care, Guangdong Geriatrics Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
Department of Intensive Care, Guangdong Geriatrics Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, No. 106 Zhongshan Road, Guangzhou 510080, China.
Open Life Sci. 2024 Jul 24;19(1):20220900. doi: 10.1515/biol-2022-0900. eCollection 2024.
Circular RNAs (circRNAs) play important roles in many human diseases. However, their role in the development of severe sepsis, a condition that remains one of the main causes of death in intensive care units, has not yet been defined. In this study, we interrogated the molecular mechanisms of circRNAs in severe sepsis. We profiled the expression levels of 5,680 circRNAs in plasma extracted from blood samples of 9 severe sepsis cases or 9 controls (male, age 78 ± 7) using the Human circRNA Array. To enrich protein-coding genes hosting severe sepsis-related circRNAs, we conducted gene ontology and pathways analyses. Out of the identified 760 differentially expressed circRNAs, 404 were upregulated while 356 were downregulated (fold change [FC] ≥2 or ≤-2, and false discovery ratio <0.05). Circ-0008285 (located in exons of ), showed significant upregulation in severe sepsis with an FC of 13.7, and Bonferroni-corrected < 0.05/5. In silico analysis identified Circ-0008285 interacting microRNAs as well as protein-coding genes. We systematically investigated the differential expression pattern of circRNAs in severe sepsis. The circRNAs we identified might serve as potential biomarkers for diagnosis and prognosis of sepsis.
环状RNA（circRNAs）在许多人类疾病中发挥着重要作用。然而，它们在严重脓毒症（一种仍是重症监护病房主要死亡原因之一的病症）发展中的作用尚未明确。在本研究中，我们探究了circRNAs在严重脓毒症中的分子机制。我们使用人类circRNA阵列分析了从9例严重脓毒症病例或9例对照（男性，年龄78±7岁）的血液样本中提取的血浆中5680种circRNAs的表达水平。为了富集与严重脓毒症相关的circRNAs的蛋白质编码基因，我们进行了基因本体论和通路分析。在鉴定出的760种差异表达的circRNAs中，404种上调，356种下调（倍数变化[FC]≥2或≤-2，错误发现率<0.05）。Circ-0008285（位于外显子中）在严重脓毒症中显著上调，FC为13.7，经Bonferroni校正的P<0.05/5。计算机分析确定了Circ-0008285相互作用的微小RNA以及蛋白质编码基因。我们系统地研究了严重脓毒症中circRNAs的差异表达模式。我们鉴定出的circRNAs可能作为脓毒症诊断和预后的潜在生物标志物。
