Nong Qian, Malviya Mukesh Kumar, Lin Li, Xie Jinlan, Mo Zhanghong, Solanki Manoj Kumar, Solanki Anjali Chandrol, Wang Zeping, Song Xiupeng, Li Yangrui, Li Changning
Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Institute of Sciences, SAGE University Indore, Indore, M.P. 452020, India.
ACS Omega. 2024 Jul 9;9(29):31684-31693. doi: 10.1021/acsomega.4c02341. eCollection 2024 Jul 23.
Exogenous hormones play a crucial role in regulating plant growth, development, and stress tolerance. However, the effects of exogenous abscisic acid (ABA) on sugarcane seedlings under water stress remain poorly understood. Here, in this study, a pot experiment was conducted on sugarcane seedlings 4 weeks after transplanting, employing three treatments: control (normal growth), drought (water stress), and drought + ABA (foliar application of 100 μM ABA before water stress). The main objectives of this research are to understand the effects of exogenous ABA on sugarcane seedlings under water stress conditions and to assess the changes in antioxidant enzyme activity and phytohormone levels in response to exogenous ABA. Water stress was induced in the solution culture by adding 25% (w/v) polyethylene glycol (PEG) 6000 to the Hoagland solution. Leaf samples were collected at 3, 6, and 9 days after treatment, and the photosynthetic and biochemical responses of ABA-treated plants to drought stress were investigated. The indole acetic acid (IAA) activity of the ABA-treated drought plants is compared to that of drought plants. Moreover, the endogenous ABA levels of the ABA-treated drought plants were significantly enhanced by 42.2, 39.9, and 42.3% at 3, 6, and 9 days, respectively, compared to those of drought plants. Additionally, the proline content of the ABA-treated drought plants significantly increased by 45 and 80% at 6 and 9 days, respectively, compared to that of drought plants. The expression of the catalase 1 () gene was increased in the ABA-treated drought plants by 2.1-fold, 0.7-fold, and 1.37-fold at 3, 6, and 9 days, respectively, compared to that in drought plants. Similarly, the expression of superoxide dismutase, peroxidase, and ascorbate peroxidase genes of the ABA-treated drought plants also increased compared to those of the drought plants. In conclusion, foliar application of ABA mitigated the negative effects of water shortage of sugarcane plants under water stress. Applying ABA improved the antioxidant defense system of sugarcane plants under drought stress, thereby enhancing their photosynthetic activities and productivity.
外源激素在调节植物生长、发育和抗逆性方面发挥着关键作用。然而，水分胁迫下外源脱落酸（ABA）对甘蔗幼苗的影响仍知之甚少。在此，本研究对移栽4周后的甘蔗幼苗进行了盆栽试验，采用三种处理：对照（正常生长）、干旱（水分胁迫）和干旱 + ABA（水分胁迫前叶面喷施100 μM ABA）。本研究的主要目的是了解水分胁迫条件下外源ABA对甘蔗幼苗的影响，并评估抗氧化酶活性和植物激素水平对外源ABA的响应变化。通过向霍格兰溶液中添加25%（w/v）聚乙二醇（PEG）6000在溶液培养中诱导水分胁迫。在处理后3、6和9天采集叶片样本，研究ABA处理的植物对干旱胁迫的光合和生化响应。将ABA处理的干旱植物的吲哚乙酸（IAA）活性与干旱植物的进行比较。此外，与干旱植物相比，ABA处理的干旱植物的内源ABA水平在3、6和9天分别显著提高了42.2%、39.9%和42.3%。此外，与干旱植物相比，ABA处理的干旱植物的脯氨酸含量在6天和9天分别显著增加了45%和80%。与干旱植物相比，ABA处理的干旱植物中过氧化氢酶1（）基因的表达在3、6和9天分别增加了2.1倍、0.7倍和1.37倍。同样，与干旱植物相比，ABA处理的干旱植物中超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶基因的表达也增加了。总之，叶面喷施ABA减轻了水分胁迫下甘蔗植株缺水的负面影响。施用ABA改善了干旱胁迫下甘蔗植株的抗氧化防御系统，从而提高了它们的光合活性和生产力。
