Gupta Akshat, Jha Puja K, Aggarwal Richa, Ahirwar Ashok K, Almeida Edelbert A, Kar Rajarshi
MBBS (Pursuing), 79317 University College of Medical Sciences and GTB Hospital , Delhi, India.
Department of Biochemistry, 79317 University College of Medical Sciences and GTB Hospital , Delhi, India.
Horm Mol Biol Clin Investig. 2024 Jul 30;45(4):149-155. doi: 10.1515/hmbci-2024-0010. eCollection 2024 Dec 1.
The underlying causes and mechanisms of pre-eclampsia (PE), its exact etiology remains unclear and poorly understood. Hypoxia, ischemia, and oxidative stress induced by free radicals have been associated with development of PE. Ischemia-modified albumin (IMA) is a chemically modified albumin due to oxidative stress. IMA, a serum biomarker of hypoxia, ischemia, and oxidative free radicals is a potential biomarker for PE. The aim of the current proposal was to study serum IMA as a diagnostic biomarker of pre-eclampsia (PE) in pregnant females and to evaluate the correlation between serum IMA and different markers of pre-eclampsia (BP, urinary protein, LFT, KFT, serum total protein & uric acid).
A total of 60 pregnant women aged between 21 and 35 years were recruited (30 PE cases and 30 normal pregnancy). Serum IMA was measured by spectrophotometric method developed by Bar-Or D. BP and biochemical parameters (urinary protein, LFT, KFT, serum total protein & uric acid) were also assayed and compared between two groups. Correlation analysis was done for analyzing the relationship between serum IMA and biochemical parameters.
The mean serum IMA was significantly higher in normotensive pregnant females (0.93 ABSU) than PE cases (0.71 ABSU). Kidney function and liver function parameters were more deranged in PE cases than in controls. Serum IMA was positively correlated with serum creatinine (r=0.322), serum uric acid (r=0.54) and urinary protein (0.376) whereas negatively correlated with total serum bilirubin (r=-0.515) and serum albumin (r=-0.380).
Elevated serum IMA concentrations in normotensive pregnant controls as compared to PE cases suggest that apart from ongoing ischemia and oxidative stress in placenta IMA values are influenced by many other mechanisms in pregnancy.
先兆子痫（PE）的潜在病因和机制，其确切病因仍不清楚且了解不足。自由基诱导的缺氧、缺血和氧化应激与PE的发生发展有关。缺血修饰白蛋白（IMA）是一种因氧化应激而化学修饰的白蛋白。IMA作为缺氧、缺血和氧化自由基的血清生物标志物，是PE的潜在生物标志物。本研究的目的是研究血清IMA作为妊娠女性先兆子痫（PE）的诊断生物标志物，并评估血清IMA与先兆子痫的不同标志物（血压、尿蛋白、肝功能、肾功能、血清总蛋白和尿酸）之间的相关性。
共招募60名年龄在21至35岁之间的孕妇（30例PE患者和30例正常妊娠者）。采用Bar-Or D开发的分光光度法测量血清IMA。还测定了两组的血压和生化参数（尿蛋白、肝功能、肾功能、血清总蛋白和尿酸）并进行比较。进行相关性分析以分析血清IMA与生化参数之间的关系。
血压正常的妊娠女性的平均血清IMA（0.93 ABSU）显著高于PE患者（0.71 ABSU）。PE患者的肾功能和肝功能参数比对照组更紊乱。血清IMA与血清肌酐（r = 0.322）、血清尿酸（r = 0.54）和尿蛋白（0.376）呈正相关，而与血清总胆红素（r = -0.515）和血清白蛋白（r = -0.380）呈负相关。
与PE患者相比，血压正常的妊娠对照组血清IMA浓度升高表明，除了胎盘持续存在的缺血和氧化应激外，IMA值还受妊娠中许多其他机制的影响。
