Department of Chemistry, University of Toronto, Toronto, Ontario, Canada M5S3M2.
Department of Chemistry, Weinberg College of Arts & Sciences, Northwestern University, Evanston, Illinois 60208 United States.
ACS Sens. 2024 Aug 23;9(8):3864-3869. doi: 10.1021/acssensors.4c00861. Epub 2024 Jul 29.
The detection of small molecules beyond glucose remains an ongoing challenge in the field of biomolecular sensing owing to their small size, diverse structures, and lack of alternative non-enzymatic sensing methods. Here, we present a new reagentless electrochemical approach for small molecule detection that involves directed movement of electroactive analytes through a self-assembled monolayer to an electrode surface. Using this method, we demonstrate detection of several physiologically relevant small molecules as well as the capacity for the system to operate in several biological fluids. We anticipate that this mechanism will further improve our capacity for small molecule measurement and provide a new generalizable monolayer-based technique for electrochemical assessment of various electroactive analytes.
由于小分子的体积小、结构多样，且缺乏替代的非酶传感方法，因此在生物分子传感领域，对其进行检测仍然是一个持续存在的挑战。在这里，我们提出了一种新的无试剂电化学小分子检测方法，该方法涉及将具有电活性的分析物通过自组装单分子层定向移动到电极表面。使用这种方法，我们证明了对几种生理相关的小分子的检测，以及该系统在几种生物流体中运行的能力。我们预计，这种机制将进一步提高我们对小分子测量的能力，并为电化学评估各种具有电活性的分析物提供一种新的可推广的基于单层的技术。
