Identification of the gene family in rice () and its response to rice blast.

To better understand RNA-binding proteins in rice, a comprehensive investigation was conducted on the gene family of rice. It encompassed genome-wide identification and exploration of its role in rice blast resistance. The physicochemical properties of the rice gene family were analyzed. There genes were also analyzed for their conserved domains, motifs, location information, gene structure, phylogenetic trees, collinearity, and cis-acting elements. Furthermore, alterations in the expression patterns of selected genes were assessed using quantitative real-time PCR (qRT-PCR). A total of 212 members of the gene family were identified, which were dispersed across 12 chromosomes. These genes all exhibit multiple exons and introns, all of which encompass the conserved RRM1 domain and share analogous motifs. This observation suggests a high degree of conservation within the encoded sequence domain of these genes. Phylogenetic analysis revealed the existence of five subfamilies within the gene family. Furthermore, investigation of the promoter region identified cis-regulatory elements that are involved in nucleic acid binding and interaction with multiple transcription factors. By employing GO and KEGG analyses, four genes were tentatively identified as crucial contributors to plant immunity, while the gene family was also found to have a significant involvement in the complex of alternative splicing. The qRT-PCR results revealed distinct temporal changes in the expression patterns of genes following rice blast infection. Additionally, gene expression analysis indicates that the majority of genes exhibited constitutive expressions. These findings enrich our understanding of the gene family. They also provide a foundation for further research on immune mechanisms rice and the management of rice blast.