Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
College of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
PeerJ. 2024 Jul 26;12:e17750. doi: 10.7717/peerj.17750. eCollection 2024.
Researchers often need to synthesize genes of interest in this era of synthetic biology. Gene synthesis by PCR assembly of multiple DNA fragments is a quick and economical method that is widely applied. Up to now, there have been a few software solutions for designing fragments in gene synthesis. However, some of these software solutions use programming languages that are not popular now, other software products are commercial or require users to visit servers. In this study, we propose a Python program to design DNA fragments for gene synthesis. The algorithm is designed to meet the experimental needs. Also, the source code with detailed annotation is freely available for all users. Furthermore, the feasibility of the algorithm and the program is validated by experiments. Our program can be useful for the design of gene synthesis in the labs and help the study of gene structure and function.
在合成生物学时代，研究人员经常需要合成感兴趣的基因。通过聚合酶链式反应（PCR）将多个 DNA 片段组装进行基因合成是一种快速且经济的方法，被广泛应用。迄今为止，已经有一些用于基因合成片段设计的软件解决方案。然而，其中一些软件解决方案使用的编程语言现在并不流行，其他一些软件产品是商业的或要求用户访问服务器。在这项研究中，我们提出了一个用于基因合成片段设计的 Python 程序。该算法旨在满足实验需求。此外，带有详细注释的源代码可供所有用户免费使用。此外，通过实验验证了算法和程序的可行性。我们的程序可用于实验室中的基因合成设计，有助于基因结构和功能的研究。
