Kuroda Hidetaka, Tsukimoto Shota, Kosai Azuma, Komatsu Noriko, Ouchi Takehito, Kimura Maki, Sato-Boku Aiji, Yoshida Ayaka, Yoshino Fumihiko, Abe Takahiro, Shibukawa Yoshiyuki, Sanuki Takuro
Department of Dental Anesthesiology, Kanagawa Dental University, Kanagawa, JPN.
Department of Oral and Maxillofacial Surgery, Kanagawa Dental University, Kanagawa, JPN.
Cureus. 2024 Jun 29;16(6):e63479. doi: 10.7759/cureus.63479. eCollection 2024 Jun.
Introduction Oxidative stress, an imbalance between reactive oxygen species (ROS) production and antioxidant defenses, plays an important role in various dental diseases. Local anesthetics are frequently used in dentistry. The potential antioxidant activity of dental local anesthetics can contribute to dental practice. Therefore, this study aimed to investigate the ROS-scavenging activities of three commonly used dental local anesthetics, lidocaine, prilocaine, and articaine, focusing on their effects on hydroxyl radicals (HO) and superoxide anions (O ). Materials and methods The electron spin resonance (ESR) spin-trapping technique was employed to specifically measure the ROS-scavenging activities of these local anesthetics at varying concentrations. Results Lidocaine, prilocaine, and articaine exhibited concentration-dependent HO-scavenging activities, with IC values of 0.029%, 0.019%, and 0.014%, respectively. Lidocaine and prilocaine showed concentration-dependent O -scavenging activity, with IC values of 0.033% and 0.057%, respectively. However, articaine did not scavenge O . Conclusions The proactive use of dental local anesthetics may mitigate oxidative injury and inflammatory damage through direct ROS scavenging. However, further research is needed to elucidate the specific mechanisms underlying the antioxidant effects of these dental local anesthetics and their potential impact on the dental diseases associated with oxidative stress.
引言 氧化应激是活性氧（ROS）生成与抗氧化防御之间的失衡，在各种牙科疾病中起重要作用。局部麻醉剂在牙科中经常使用。牙科局部麻醉剂的潜在抗氧化活性有助于牙科实践。因此，本研究旨在研究三种常用牙科局部麻醉剂利多卡因、丙胺卡因和阿替卡因的ROS清除活性，重点关注它们对羟基自由基（HO）和超氧阴离子（O）的影响。材料和方法 采用电子自旋共振（ESR）自旋捕获技术，特异性测量这些局部麻醉剂在不同浓度下的ROS清除活性。结果 利多卡因、丙胺卡因和阿替卡因表现出浓度依赖性的HO清除活性，IC值分别为0.029%、0.019%和0.014%。利多卡因和丙胺卡因表现出浓度依赖性的O清除活性，IC值分别为0.033%和0.057%。然而，阿替卡因不能清除O。结论 积极使用牙科局部麻醉剂可能通过直接清除ROS减轻氧化损伤和炎症损伤。然而，需要进一步研究以阐明这些牙科局部麻醉剂抗氧化作用的具体机制及其对与氧化应激相关的牙科疾病的潜在影响。
