Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo, 11795, Egypt.
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo, 11795, Egypt.
Chem Biodivers. 2024 Nov;21(11):e202400959. doi: 10.1002/cbdv.202400959. Epub 2024 Sep 16.
Genus Asparagus is well known for its pharmacological activities and ethnopharmacological applications. In folk medicine, it is used in the management of several diseases such as diabetes, inflammations, and rheumatism. This work aimed to investigate the potential of Asparagus densiflorus meyeri root & aerial parts extracts as cytotoxic and anti-inflammatory and the investigation of their chemical profile. GC analysis & Folin-Ciocalteu and gravimetric methods were used respectively to estimate the lipoidal, phenolic, and saponin contents. MTT assay was conducted using two cell lines (MCF-7 & HepG2) to investigate the cytotoxic and anti-inflammatory activity using TNF-α stimulated MCF-7 cells through monitoring the level of nitric oxide release and NF-κB gene expression. Preliminary phytochemical investigation indicated that both extracted parts are equally rich in saponins, flavonoids, carbohydrates and/or glycosides, and sterols and/or triterpenes. Palmitic acid and β sitosterol represented the major saturated fatty acids and sterol, respectively. A significant cytotoxic activity against MCF-7 cells was recorded for DCM extract (IC 26.13 μg/ml). All tested extracts showed a significant decrease in NO release and NF-κB gene expression thus it possesses a potential anti-inflammatory activity. A. densiflorus meyeri is considered a good candidate as a food supplement for protection from malignancy.
天门冬属因其药理学活性和民族药理学应用而广为人知。在民间医学中，它被用于治疗多种疾病，如糖尿病、炎症和风湿病。本工作旨在研究密花天门冬根和地上部分提取物的潜在细胞毒性和抗炎作用，并研究其化学特征。分别采用 GC 分析、福林-考尔法和重量法来估计脂质、酚类和皂苷含量。采用 MTT 法，通过监测一氧化氮释放和 NF-κB 基因表达水平，使用 TNF-α 刺激的 MCF-7 细胞，研究两种细胞系（MCF-7 和 HepG2）的细胞毒性和抗炎活性。初步的植物化学研究表明，两个提取部分都富含皂苷、黄酮类、碳水化合物和/或糖苷以及甾醇和/或三萜类化合物。棕榈酸和 β-谷甾醇分别代表主要的饱和脂肪酸和甾醇。DCM 提取物对 MCF-7 细胞具有显著的细胞毒性（IC 26.13 μg/ml）。所有测试的提取物均能显著降低 NO 释放和 NF-κB 基因表达，因此具有潜在的抗炎活性。密花天门冬被认为是一种很好的候选食品补充剂，可用于预防恶性肿瘤。
