Berdaguer Roland, van der Wielen Nicky, Lorenzo Zulema Carracedo, Testerink Christa, Karlova Rumyana
Laboratory of Plant Physiology, Wageningen University, Wageningen, Netherlands.
Laboratory of Entomology, Wageningen University, Wageningen, Netherlands.
Plant Cell Environ. 2024 Dec;47(12):4754-4767. doi: 10.1111/pce.15063. Epub 2024 Jul 30.
The roots of vascular plants are colonised by a multitude of microbes, which play an important role in plant health and stress resilience. Drought stress in particular is devastating for crop yield and causes major shifts in the rhizosphere microbial communities. However, the microbiome associated to the rhizoids (hereafter termed rhizoid-sphere) of the nonvascular bryophytes remains largely unexplored. Here, we use amplicon sequencing to explore the rhizoid-sphere microbiome of three bryophyte species under drought and well-watered conditions. Comparing rhizoid-sphere microbial communities associated with the two liverworts Marchantia polymorpha and Marchantia paleacea and the moss Physcomitrium patens showed characteristic differences in composition between host species and both conserved and unique changes under drought. At phylum level, these changes were similar to changes in the rhizosphere of angiosperms under drought. Furthermore, we observed strong differences in rhizoid-sphere colonisation between bryophyte species for taxa known for nitrogen fixation and plant growth promotion. Interestingly, M. polymorpha prioritised the growth of belowground organs under osmotic stress, as is the case for angiosperms under drought. Taken together, our results show interesting parallels between bryophytes and angiosperms in the relation with their rhizo(id-)sphere, suggesting evolutionary conservation among land plants in their response to drought stress.
维管植物的根系被大量微生物定殖，这些微生物对植物健康和抗逆性起着重要作用。特别是干旱胁迫对作物产量具有毁灭性影响，并导致根际微生物群落发生重大变化。然而，与非维管苔藓植物的假根（以下称为假根球）相关的微生物组在很大程度上仍未得到探索。在这里，我们使用扩增子测序来探究三种苔藓植物在干旱和水分充足条件下的假根球微生物组。比较与两种地钱（多歧苔和苍白苔）以及苔藓立碗藓相关的假根球微生物群落，结果显示宿主物种之间在组成上存在特征差异，并且在干旱条件下既有保守变化也有独特变化。在门水平上，这些变化与被子植物根际在干旱条件下的变化相似。此外，我们观察到，对于以固氮和促进植物生长而闻名的类群，苔藓植物物种之间在假根球定殖方面存在显著差异。有趣的是，多歧苔在渗透胁迫下优先促进地下器官的生长，这与被子植物在干旱条件下的情况相同。综上所述，我们的结果表明苔藓植物和被子植物在与其根（假根）球的关系方面存在有趣的相似之处，这表明陆地植物在对干旱胁迫的反应方面具有进化保守性。
