Park Hye Yoon, Buxbaum Adina R, Singer Robert H
Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, USA.
Methods Enzymol. 2010;472:387-406. doi: 10.1016/S0076-6879(10)72003-6.
Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.
mRNA的不对称分布是在多种细胞类型中观察到的普遍现象。mRNA的转录后移动和定位提供了一种重要机制，可将某些蛋白质靶向到其发挥功能的特定细胞质区域。最近的技术进步使得在活细胞中实时可视化单个mRNA分子成为可能。分析单个mRNA运动的研究揭示了复杂的RNA转运系统。本章概述了单粒子追踪的一般方法以及用于单mRNA检测的一些方法。
