Department of Fisheries Resource Management, FRHPHM division, ICAR-Central Institute of Fisheries Education, Mumbai, 400061, India.
Department of Fisheries Resource Management, College of Fisheries, Assam Agricultural University, Raha, 782103, India.
Mol Biol Rep. 2024 Jul 30;51(1):875. doi: 10.1007/s11033-024-09825-7.
The Northeast India, being part of two global biodiversity hotspot namely the Indo-Burma and Eastern Himalayan Hotspots supports a wide variety of rich aquatic biodiversity including fishes. The family Danionidae is a widely diverse group inhabiting the upper colder stretches of river although few are abundant in the lower stretches. The persisting similarity in the morphological appearance and body colouration within the members of this family seeks an integrated method to identify the species correctly.
In the present study, the mt-DNA barcode was generated for correct identification and confirmation of the species. A total of nine mitochondrial cytochrome c oxidase subunit I gene sequences were generated for each species under the study. The pairwise distance values ranged from 0.09 to 9.11% within species and 9.06-32.71% between species. A neighbour-joining tree was constructed based on the Kimura 2 parameter model. Two major groups were observed where Danioninae formed a sister group to the Chedrinae and Rasborinae.
The present study is a preliminary work to document and identify the species under the family Danionidae from Brahmaputra basin, Assam, using molecular tools and establish the phylogenetic relationship.
印度东北部是两个全球生物多样性热点地区之一的印度-缅甸和东喜马拉雅热点地区的一部分，拥有广泛的丰富水生生物多样性，包括鱼类。Danionidae 科是一个广泛多样的群体，栖息在上部较冷的河流段，尽管少数在下部较丰富。该家族成员在形态外观和体色上的持续相似性需要一种综合的方法来正确识别物种。
在本研究中，生成了 mt-DNA 条码以进行正确识别和确认物种。为研究中的每个物种生成了总共九个线粒体细胞色素 c 氧化酶亚基 I 基因序列。种内的成对距离值范围为 0.09%至 9.11%，种间为 9.06%至 32.71%。基于 Kimura 2 参数模型构建了一个邻接树。观察到两个主要的组，其中 Danioninae 与 Chedrinae 和 Rasborinae 形成姐妹群。
本研究是使用分子工具对来自阿萨姆邦布拉马普特拉河流域的 Danionidae 科物种进行记录和识别的初步工作，并建立了系统发育关系。
